de Moura-Leal Adinaide C, de Sousa João Macedo, de Moura-Leal Heyder, de Sousa Gabriela V, Alencar Airlane P, Borges Umbelina Soares, Pinto Giovanny R, da Silva Benedito B
a Department of Gynecology , Federal University of Piauí , Teresina, Piauí , Brazil .
b Veterinary Hospital, Federal University of Piauí , Teresina, Piauí , Brazil .
Gynecol Endocrinol. 2016 Jun;32(6):453-6. doi: 10.3109/09513590.2015.1128408. Epub 2016 Jan 6.
The aim of this study was to evaluate the effects of raloxifene and tamoxifen on Ki-67 antigen expression in the vaginal epithelium of castrated rats.
Thirty-nine virgin, adult, castrated female Wistar-Hannover rats were randomly divided into three groups: Group I (control, n = 13), Group II (raloxifene, n = 13) and Group III (tamoxifen, n = 13). After confirmation of their hypoestrogenic state, the rats were given 0.5 ml of propylene glycol (vehicle), 750 μg of raloxifene or 250 μg of tamoxifen, respectively, by gavage, for 30 days. On the 31st day, the rats were euthanized and their vaginas removed and fixed in 10% buffered formalin for of Ki-67 immunohistochemical evaluation. Data were analyzed using Levene's test and Tukey's method (p < 0.05).
Mean Ki-67 expression in groups I, II and III was 27 ± 2.6, 32.3 ± 1.9 and 43.7 ± 3.5, respectively. In Group III (tamoxifen), there was a greater proportion of stained cells compared to Groups I and II (p < 0.0003), with no statistically significant difference between Groups I and II (p = 0.3626).
The present results show that tamoxifen significantly increased cell proliferation in the vaginal epithelium of the castrated rats and no difference between the raloxifene and control groups.
本研究旨在评估雷洛昔芬和他莫昔芬对去势大鼠阴道上皮中Ki-67抗原表达的影响。
39只成年未孕去势雌性Wistar-Hannover大鼠随机分为三组：第一组（对照组，n = 13）、第二组（雷洛昔芬组，n = 13）和第三组（他莫昔芬组，n = 13）。在确认其低雌激素状态后，分别通过灌胃给予大鼠0.5 ml丙二醇（赋形剂）、750 μg雷洛昔芬或250 μg他莫昔芬，持续30天。在第31天，将大鼠安乐死，取出其阴道并固定于10%缓冲福尔马林中，用于Ki-67免疫组织化学评估。数据采用Levene检验和Tukey方法进行分析（p < 0.05）。
第一组、第二组和第三组的平均Ki-67表达分别为27±2.6、32.3±1.9和43.7±3.5。与第一组和第二组相比，第三组（他莫昔芬组）染色细胞的比例更高（p < 0.0003），而第一组和第二组之间无统计学显著差异（p = 0.3626）。
目前的结果表明，他莫昔芬显著增加了去势大鼠阴道上皮中的细胞增殖，而雷洛昔芬组与对照组之间无差异。
