小鼠骨髓瘤细胞的抗原结合突变体。
Antigen-binding mutants of mouse myeloma cells.
作者信息
Cook W D, Scharff M D
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5687-91. doi: 10.1073/pnas.74.12.5687.
Abstract
A cultured mouse myeloma cell line, S107, that secretes an IgA phosphocholine-binding immunoglobulin has been cloned in soft agar and overlaid with phosphocholine-hemocyanin. Spontaneous mutants that secrete immunoglobulin with a decreased ability to precipitate antigen were detected with this plate assay and occur at a very high frequency. From one such mutant, phenotypic revertants arise spontaneously with a frequency of 0.28-2.8%. This mutant and one of its revertants were studied, and they were found to differ from the parent and from each other serologically and in antigen binding. While it is not yet clear whether these findings bear any relationship to the normal generation of antibody diversity, they do indicate that it is possible to generate antigen binding diversity in somatic cells.
摘要
一种分泌IgA磷酸胆碱结合免疫球蛋白的培养小鼠骨髓瘤细胞系S107已在软琼脂中克隆，并覆盖有磷酸胆碱-血蓝蛋白。通过该平板试验检测到分泌沉淀抗原能力降低的免疫球蛋白的自发突变体，其出现频率非常高。从一个这样的突变体中，表型回复体以0.28 - 2.8%的频率自发产生。对该突变体及其一个回复体进行了研究，发现它们在血清学和抗原结合方面与亲本以及彼此都存在差异。虽然目前尚不清楚这些发现是否与抗体多样性的正常产生有任何关系，但它们确实表明在体细胞中产生抗原结合多样性是可能的。
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本文引用的文献
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An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity.本斯·琼斯蛋白和骨髓瘤轻链可变区序列分析及其对抗体互补性的影响。
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