一种结合磷酸胆碱的小鼠免疫球蛋白Fab的三维结构及抗原结合位点的性质。
The three-dimensional structure of a phosphorylcholine-binding mouse immunoglobulin Fab and the nature of the antigen binding site.
作者信息
Segal D M, Padlan E A, Cohen G H, Rudikoff S, Potter M, Davies D R
出版信息
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4298-302. doi: 10.1073/pnas.71.11.4298.
Abstract
The structure of the Fab of McPC 603, a mouse myeloma protein with phosphorylcholine binding activity, has been determined to 3.1-A resoltuion. The four domains are found to be structurally similar with a well-defined double-layer structure. A large cavity exists at one end of the fragment, the walls of which are formed exclusively of hypervariable residues. Phosphorylcholine binds in this cavity and forms specific interactions with several well-defined amino-acid side chains of the protein. The hapten is bound asymmetrically and interacts more with the heavy chain than with the light chain.
摘要
具有磷酸胆碱结合活性的小鼠骨髓瘤蛋白McPC 603的Fab片段结构已确定，分辨率达3.1埃。发现四个结构域在结构上相似，具有明确的双层结构。片段一端存在一个大腔，其壁完全由高变残基形成。磷酸胆碱结合在这个腔内，并与蛋白质的几个明确的氨基酸侧链形成特异性相互作用。半抗原以不对称方式结合，与重链的相互作用比对轻链的更多。
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本文引用的文献
1
The matching of physical models to three-dimensional electron-density maps: a simple optical device.物理模型与三维电子密度图的匹配：一种简单的光学装置。
J Mol Biol. 1968 Oct 14;37(1):225-30. doi: 10.1016/0022-2836(68)90085-5.
2
Stereochemical criteria for polypeptides and proteins. V. Conformation of a system of three linked peptide units.多肽和蛋白质的立体化学标准。V. 三个相连肽单元系统的构象
Biopolymers. 1968 Oct;6(10):1425-36. doi: 10.1002/bip.1968.360061006.
3
An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity.本斯·琼斯蛋白和骨髓瘤轻链可变区序列分析及其对抗体互补性的影响。
J Exp Med. 1970 Aug 1;132(2):211-50. doi: 10.1084/jem.132.2.211.
4
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5
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Eur J Biochem. 1974 Jan 3;41(1):91-6. doi: 10.1111/j.1432-1033.1974.tb03247.x.
6
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Eur J Biochem. 1974 Jun 15;45(2):513-24. doi: 10.1111/j.1432-1033.1974.tb03576.x.
7
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