Schwartz N B, Channing C P
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5721-4. doi: 10.1073/pnas.74.12.5721.
The present studies were carried out to see if porcine follicular fluid could inhibit increases in serum follicle stimulating hormone (FSH) levels when injected into the rat. For these studies the pentobarbital-treated proestrous rat was chosen as the major test animal model. If an artificial surge of luteinizing hormone (LH) is administered to these rats, it can induce a synchronized secondary rise in FSH secretion rate. Normal saline-treated rats were also used as test animals. They exhibit preovulatory endogenous "surges" of LH and FSH, and also a secondary FSH rise. Porcine follicular fluid was harvested from medium-sized and large (3- to 10-mm diameter) follicles and treated with charcoal to remove endogenous steroids. Charcoal-treated porcine serum served as a control solution. The fluid was injected intraperitoneally in two 0.5-ml doses into pentobarbital-treated proestrous rats immediately and 3 hr after LH injection. Follicular fluid, but not the serum, suppressed the secondary, LH-induced FSH rise (P < 0.01) in a dose-dependent manner, without altering the effects of LH upon serum ovarian steroid levels or follicular rupture. It was effective down to a total dose of 200 mul. Porcine follicular fluid also blocked the secondary FSH surge in normal proestrous rats exhibiting endogenous LH/FSH primary surges. Thus, it would appear that porcine follicular fluid contains a non-steroidal substance(s) that can block the secretion of FSH that is secondary to a natural or artificial LH surge.
本研究旨在观察将猪卵泡液注射到大鼠体内时，是否能抑制血清卵泡刺激素（FSH）水平的升高。在这些研究中，选择经戊巴比妥处理的动情前期大鼠作为主要实验动物模型。如果对这些大鼠给予促黄体生成素（LH）的人工激增，可诱导FSH分泌率同步出现继发性升高。生理盐水处理的大鼠也用作实验动物。它们表现出排卵前LH和FSH的内源性“激增”，以及FSH的继发性升高。从直径3至10毫米的中型和大型卵泡中采集猪卵泡液，并用活性炭处理以去除内源性类固醇。经活性炭处理的猪血清用作对照溶液。将该液体以两个0.5毫升剂量腹腔内注射到经戊巴比妥处理的动情前期大鼠体内，分别在LH注射后立即和3小时注射。卵泡液而非血清以剂量依赖方式抑制了LH诱导的FSH继发性升高（P<0.01），且不改变LH对血清卵巢类固醇水平或卵泡破裂的影响。总剂量低至200微升时仍有效。猪卵泡液还阻断了表现出内源性LH/FSH初次激增的正常动情前期大鼠的FSH继发性激增。因此，似乎猪卵泡液含有一种非甾体物质，可阻断继发于自然或人工LH激增后的FSH分泌。
