Suppression of pituitary secretion of follicle-stimulating hormone by porcine follicular fluid during pro-oestrus and oestrus in the rat: effects on gonadotrophin and steroid secretion, follicular development and ovulation during the following cycle.

In this study, we have examined whether the suppression of raised plasma FSH concentrations at pro-oestrus and/or oestrus by porcine follicular fluid (PFF) affected the development of follicles for ovulation in the next cycle. Adult, 4-day-cyclic rats were injected with PFF or pig serum at various hours of pro-oestrus and oestrus. Plasma FSH levels were suppressed following PFF treatment at any time of pro-oestrus and oestrus. Furthermore, this suppression was always followed by a 'rebound' increase in plasma FSH. In contrast, plasma LH concentrations were unaffected by PFF treatment and neither gonadotrophin was altered by treatment with pig serum. When rats treated with PFF or pig serum were allowed to complete one additional cycle, plasma LH and FSH concentrations at the pro-oestrus and oestrus after treatment were not significantly different among groups regardless of treatment or time of treatment. All ovaries of rats treated with PFF or pig serum on the next pro-oestrus morning before ovulation were histologically similar. Furthermore, all animals ovulated a normal complement of ova at the next oestrus regardless of whether preovulatory, secondary or both increases of plasma FSH had been blocked by PFF treatment during the previous cycle. However, animals given PFF during the preceding cycle, plasma oestradiol and progesterone concentrations were significantly altered on the morning and afternoon of pro-oestrus respectively. These results suggest that increased plasma FSH concentrations at pro-oestrus and oestrus may not be essential for folliculogenesis and ovulation in the subsequent cycle. Alternatively, the 'rebound' of FSH on day 1 of dioestrus after the suppression of both phases of FSH secretion at pro-oestrus and oestrus may be sufficient to provide ovulatory follicles for the next pro-oestrous day.