DePaolo L V, Hirshfield A N, Anderson L D, Barraclough C A, Channing C P
J Endocrinol. 1979 Dec;83(3):355-68. doi: 10.1677/joe.0.0830355.
In this study, we have examined whether the suppression of raised plasma FSH concentrations at pro-oestrus and/or oestrus by porcine follicular fluid (PFF) affected the development of follicles for ovulation in the next cycle. Adult, 4-day-cyclic rats were injected with PFF or pig serum at various hours of pro-oestrus and oestrus. Plasma FSH levels were suppressed following PFF treatment at any time of pro-oestrus and oestrus. Furthermore, this suppression was always followed by a 'rebound' increase in plasma FSH. In contrast, plasma LH concentrations were unaffected by PFF treatment and neither gonadotrophin was altered by treatment with pig serum. When rats treated with PFF or pig serum were allowed to complete one additional cycle, plasma LH and FSH concentrations at the pro-oestrus and oestrus after treatment were not significantly different among groups regardless of treatment or time of treatment. All ovaries of rats treated with PFF or pig serum on the next pro-oestrus morning before ovulation were histologically similar. Furthermore, all animals ovulated a normal complement of ova at the next oestrus regardless of whether preovulatory, secondary or both increases of plasma FSH had been blocked by PFF treatment during the previous cycle. However, animals given PFF during the preceding cycle, plasma oestradiol and progesterone concentrations were significantly altered on the morning and afternoon of pro-oestrus respectively. These results suggest that increased plasma FSH concentrations at pro-oestrus and oestrus may not be essential for folliculogenesis and ovulation in the subsequent cycle. Alternatively, the 'rebound' of FSH on day 1 of dioestrus after the suppression of both phases of FSH secretion at pro-oestrus and oestrus may be sufficient to provide ovulatory follicles for the next pro-oestrous day.
在本研究中，我们检测了猪卵泡液（PFF）对动情前期和/或发情期升高的血浆促卵泡激素（FSH）浓度的抑制作用是否会影响下一个周期中用于排卵的卵泡发育。在动情前期和发情期的不同时间，给成年的、处于4天周期的大鼠注射PFF或猪血清。在动情前期和发情期的任何时间进行PFF处理后，血浆FSH水平均受到抑制。此外，这种抑制之后总是伴随着血浆FSH的“反弹”升高。相比之下，血浆促黄体生成素（LH）浓度不受PFF处理的影响，且两种促性腺激素均未因猪血清处理而改变。当用PFF或猪血清处理的大鼠再完成一个周期时，无论处理方式或处理时间如何，处理后动情前期和发情期的血浆LH和FSH浓度在各组之间均无显著差异。在排卵前的下一个动情前期早晨，用PFF或猪血清处理的大鼠的所有卵巢在组织学上相似。此外，所有动物在下一个发情期均排出正常数量的卵子，无论在前一个周期中PFF处理是否阻断了排卵前、第二次或两次血浆FSH的升高。然而，在前一个周期给予PFF的动物，在动情前期的上午和下午，血浆雌二醇和孕酮浓度分别发生了显著变化。这些结果表明，动情前期和发情期血浆FSH浓度的升高可能对于后续周期的卵泡发生和排卵并非必不可少。或者，在动情前期和发情期FSH分泌的两个阶段受到抑制后，在动情后期第1天FSH的“反弹”可能足以在下一个动情前期提供排卵卵泡。
