艾灸对内脏高敏大鼠脊髓背角NMDA受体通路调节机制的研究

Study on the Mechanism Underlying the Regulation of the NMDA Receptor Pathway in Spinal Dorsal Horns of Visceral Hypersensitivity Rats by Moxibustion.

作者信息

Wang L D, Zhao J M, Huang R J, Tan L Y, Hu Z H, Weng Z J, Wang K, Wu H G, Liu H R

机构信息

Key Laboratory of Acupuncture and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, 650 South Wanping Road, Shanghai 200030, China.

Shanghai TCM-Integrated Hospital, Shanghai University of TCM, Shanghai 200082, China.

出版信息

Evid Based Complement Alternat Med. 2016;2016:3174608. doi: 10.1155/2016/3174608. Epub 2016 Apr 20.

DOI:10.1155/2016/3174608

PMID:27200098

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4855029/

Abstract

Visceral hypersensitivity is enhanced in irritable bowel syndrome (IBS) patients. Treatment of IBS visceral pain by moxibustion methods has a long history and rich clinical experience. In the clinic, moxibustion on the Tianshu (ST25) and Shangjuxu (ST37) acupoints can effectively treat bowel disease with visceral pain and diarrhea symptoms. To investigate the regulatory function of moxibustion on the Tianshu (ST25) and Shangjuxu (ST37) acupoints on spinal cord NR1, NR2B, and PKCε protein and mRNA expression in irritable bowel syndrome (IBS) visceral hypersensitivity rats, we did some research. In the study, we found that moxibustion effectively relieved the IBS visceral hyperalgesia status of rats. Analgesic effect of moxibustion was similar to intrathecal injection of Ro 25-6981. The expression of NR1, NR2B, and PKCε in the spinal dorsal horns of IBS visceral hyperalgesia rats increased. Moxibustion on the Tianshu and Shangjuxu acupoints might inhibit the visceral hypersensitivity, simultaneously decreasing the expression of NR1, NR2B, and PKCε in spinal cord of IBS visceral hyperalgesia rats. Based on the above experimental results, we hypothesized NR1, NR2B, and PKCε of spinal cord could play an important role in moxibustion inhibiting the process of central sensitization and visceral hyperalgesia state.

摘要

内脏高敏感性在肠易激综合征（IBS）患者中增强。艾灸疗法治疗IBS内脏痛有着悠久的历史和丰富的临床经验。在临床上，艾灸天枢穴（ST25）和上巨虚穴（ST37）可有效治疗伴有内脏痛和腹泻症状的肠道疾病。为了研究艾灸天枢穴（ST25）和上巨虚穴（ST37）对肠易激综合征（IBS）内脏高敏感性大鼠脊髓NR1、NR2B和PKCε蛋白及mRNA表达的调节作用，我们开展了一些研究。在该研究中，我们发现艾灸可有效缓解大鼠的IBS内脏痛觉过敏状态。艾灸的镇痛效果与鞘内注射Ro 25 - 6981相似。IBS内脏痛觉过敏大鼠脊髓背角中NR1、NR2B和PKCε的表达增加。艾灸天枢穴和上巨虚穴可能抑制内脏高敏感性，同时降低IBS内脏痛觉过敏大鼠脊髓中NR1、NR2B和PKCε的表达。基于上述实验结果，我们推测脊髓中的NR1、NR2B和PKCε可能在艾灸抑制中枢敏化和内脏痛觉过敏状态的过程中发挥重要作用。