艾灸对慢性内脏痛觉过敏大鼠内啡肽和强啡肽的影响。
Effects of moxibustion on dynorphin and endomorphin in rats with chronic visceral hyperalgesia.
机构信息
Key Laboratory of Acupuncture-Moxibustion and Immunological Effects, Shanghai University of Traditional Chinese Medicine, 650 South Wanping Road, Xu Hui District, Shanghai, China.
出版信息
World J Gastroenterol. 2010 Aug 28;16(32):4079-83. doi: 10.3748/wjg.v16.i32.4079.
AIM
To observe the analgesic effects of moxibustion in rats with chronic visceral hyperalgesia and its influence on the concentration of dynorphin (Dyn) and endomorphin (EM) in spinal cord.
METHODS
The rat model of chronic visceral hyperalgesia was established by colorectal distention (CRD). In moxibustion (MX) group, moxibustion was applied once daily for 7 d; in sham moxibustion (SM) group, moxibustion was given to the same acupoints but with the non-smoldered end of the moxa stick. Model control (MC) group and normal control group were also studied. The scoring system of abdominal withdrawal reflex was used to evaluate visceral pain for behavioral assessment. Enzyme linked immunosorbent assay was performed to determine the concentrations of Dyn and EM in spinal cord.
RESULTS
Moxibustion significantly decreased visceral pain to CRD in this rat model, and no significant difference was detected between the SM group and the MC group. In MX group, moxibustion also increased the concentrations of Dyn and EM in spinal cord, and no significant difference was found between the SM group and the MC group.
CONCLUSION
Moxibustion therapy can significantly enhance the pain threshold of rats with chronic visceral hyperalgesia, and the effect may be closely related to the increased concentration of Dyn and EM in spinal cord.
目的
观察艾灸对慢性内脏痛大鼠的镇痛作用及其对脊髓内强啡肽（Dyn）和内吗啡肽（EM）浓度的影响。
方法
采用结直肠扩张（CRD）法建立慢性内脏痛大鼠模型。艾灸组（MX 组）每日艾灸 1 次，共 7d；假艾灸组（SM 组）给予相同穴位但艾条未燃烧的艾灸；模型对照组（MC 组）和正常对照组（NC 组）也进行了研究。采用腹壁退缩反射评分系统评估内脏痛行为。采用酶联免疫吸附法测定脊髓中 Dyn 和 EM 的浓度。
结果
艾灸显著降低了该大鼠模型对 CRD 的内脏痛觉，SM 组与 MC 组之间无显著差异。在 MX 组中，艾灸还增加了脊髓中 Dyn 和 EM 的浓度，SM 组与 MC 组之间无显著差异。
结论
艾灸疗法能显著提高慢性内脏痛大鼠的痛阈，其作用可能与脊髓内 Dyn 和 EM 浓度的增加密切相关。
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