Salahshoor Mohammad Reza, Haghjoo Mojdeh, Roshankhah Shiva, Makalani Fatemeh, Jalili Cyrus
Department of Anatomical Sciences, Medical School, Kermanshah University of Medical Sciences, Kermanshah, Iran.
Adv Biomed Res. 2018 Jan 30;7:18. doi: 10.4103/abr.abr_69_17. eCollection 2018.
Thymoquinone as the main active component of might have a various pharmacological effects such as antiapoptotic and antioxidant. Morphine is commonly used for the treatment of severe pain that can increase the generation of free radicals and affects the spermatogenesis. This study was designed to evaluate protective effects of thymoquinone against morphine-induced damages, sperm viability, count, motility, morphology and testis histology, and nitric oxide and testosterone hormone of the mice.
In this experimental study, we divided 48 mice into eight groups ( = 6); various doses of thymoquinone (2, 10, and 20 mg/kg) and morphine (20 mg/kg) plus thymoquinone (2, 10, and 20 mg/kg) were administered intraperitoneally to 48 male mice for 30 consequent days. Male reproductive parameters including testis weight, testosterone hormone, serum nitric oxide, germinal thickness, sperm morphology, count, viability, and motility were analyzed and compared.
The results indicated that morphine administration significantly decreased germinal thickness, testis weight, testosterone level, viability, morphology, count, and motility of sperm and increased nitric oxide as compared to saline group ( < 0.05). However, increasing the dose of thymoquinone in the thymoquinone and thymoquinone plus morphine groups significantly decreases nitric oxide level ( < 0.05) while significantly boosted motility, morphology, count, viability of sperm cells, germinal thickness, and testosterone hormone in all groups as compared to morphine group ( < 0.05).
It seems that thymoquinone administration could increase the quality some of spermatozoa and improves morphine-induced adverse effects on reproductive parameters in male mice.
百里醌作为[具体物质]的主要活性成分，可能具有多种药理作用，如抗凋亡和抗氧化作用。吗啡常用于治疗剧痛，但其会增加自由基的产生并影响精子发生。本研究旨在评估百里醌对吗啡诱导的损伤、小鼠精子活力、数量、运动能力、形态以及睾丸组织学、一氧化氮和睾酮激素的保护作用。
在本实验研究中，我们将48只小鼠分为八组（每组n = 6）；连续30天对48只雄性小鼠腹腔注射不同剂量的百里醌（2、10和20 mg/kg）以及吗啡（20 mg/kg）加百里醌（2、10和20 mg/kg）。分析并比较雄性生殖参数，包括睾丸重量、睾酮激素、血清一氧化氮、生精厚度、精子形态、数量、活力和运动能力。
结果表明，与生理盐水组相比，给予吗啡显著降低了生精厚度、睾丸重量、睾酮水平、精子活力、形态、数量和运动能力，并增加了一氧化氮水平（P < 0.05）。然而，与吗啡组相比，在百里醌组和百里醌加吗啡组中增加百里醌剂量可显著降低一氧化氮水平（P < 0.05），同时显著提高所有组中精子细胞的运动能力、形态、数量、活力、生精厚度和睾酮激素水平（P < 0.05）。
似乎给予百里醌可以提高部分精子的质量，并改善吗啡对雄性小鼠生殖参数的不良影响。
