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[大鼠大脑皮层“暗”神经元和“亮”神经元能量代谢酶活性的电镜细胞化学研究]

[Electron microscopic-cytochemical study of the enzyme activity of energy metabolism in "dark" and "light" neurons of the rat cerebral cortex].

作者信息

Solov'eva Zh F, Esipova Z Ia

出版信息

Biull Eksp Biol Med. 1989 Apr;107(4):475-7.

PMID:2720166
Abstract

The ultrastructural localization of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH) activity in "dark" and "light" neurons of the intact rat's frontal brain cortex has been studied. The enzymes' activity was detected with using potassium ferricyanide as artificial acceptor of electrons. In the "light" cells SDH activity is localized in the mitochondria and plasma membranes. LDH activity is localized in the mitochondria, plasma membranes and hyaloplasm. SDH and LDH activity was not found in the "dark" cells.

摘要

对完整大鼠额叶脑皮质“暗”神经元和“亮”神经元中琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)活性的超微结构定位进行了研究。使用铁氰化钾作为人工电子受体检测酶活性。在“亮”细胞中,SDH活性定位于线粒体和质膜。LDH活性定位于线粒体、质膜和胞质溶胶。在“暗”细胞中未发现SDH和LDH活性。

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