Nott D M, Grime S J, Yates J, Day D W, Baxter J N, Jenkins S A, Cooke T G
University Department of Surgery, Royal Liverpool Hospital, UK.
Br J Surg. 1989 Mar;76(3):259-63. doi: 10.1002/bjs.1800760315.
A model of microscopic liver tumour has been developed in the Fisher rat by intraportal injection of 1.6 x 10(7) Walker 256 carcinosarcoma cells. Rats were studied at 2, 4 and 6 days after the inoculation of live Walker cells. A control group received dead Walker cells. No tumour was visible in control groups at 2, 4 and 6 days after inoculation. Similarly in rats injected with live cells no tumour was visible at 2 days after inoculation but at 4 and 6 days the percentage hepatic replacement was (mean +/- s.d.) 7.0 +/- 2.3 and 27.9 +/- 6.80 respectively. The hepatic perfusion index was significantly raised at 4 and 6 days after inoculation of live cells compared with control animals and those receiving viable cells after 2 days inoculation. Portal flow and portal venous inflow were significantly reduced when the hepatic perfusion index increased but hepatic arterial flow did not alter. Changes in the hepatic haemodynamics were accompanied by increases in the portal and splanchnic vascular resistance and an increase in the amount of arteriovenous shunting through the liver. These findings confirm studies that the hepatic perfusion index is useful in the detection of occult liver metastases but that the change is not a consequence of an increase in the hepatic arterial flow.
通过门静脉注射1.6×10⁷个沃克256癌肉瘤细胞，在Fisher大鼠中建立了微观肝肿瘤模型。在接种活的沃克细胞后的第2、4和6天对大鼠进行研究。一个对照组接受死的沃克细胞。接种后第2、4和6天，对照组均未见肿瘤。同样，在注射活细胞的大鼠中，接种后第2天未见肿瘤，但在第4天和第6天，肝脏替代百分比（均值±标准差）分别为7.0±2.3和27.9±6.80。与对照动物以及接种2天后接受活细胞的动物相比，接种活细胞后第4天和第6天肝脏灌注指数显著升高。当肝脏灌注指数增加时，门静脉血流和门静脉流入量显著减少，但肝动脉血流未改变。肝脏血流动力学的变化伴随着门静脉和内脏血管阻力的增加以及通过肝脏的动静脉分流数量的增加。这些发现证实了相关研究，即肝脏灌注指数在检测隐匿性肝转移方面是有用的，但这种变化并非肝动脉血流增加的结果。
