Drescher D G, Khan K M, Arden R L, Drescher M J, Kerr T P, Hatfield J S
Department of Otolaryngology, Wayne State University School of Medicine, Detroit, MI 48201.
Brain Res. 1989 Apr 24;485(2):225-35. doi: 10.1016/0006-8993(89)90565-9.
A protein has been detected that is associated with the saccular hair cell layer of the rainbow trout, Salmo gairdnerii R. By one- and two-dimensional SDS polyacrylamide gel electrophoresis, the molecular weight and isoelectric point of this protein are estimated to be 13.6 and 8.8 kDa, respectively. The 13.6 kDa protein cannot be detected electrophoretically in brain, gill, liver, and fractions containing the basal lamina, non-sensory epithelium, and saccular nerve. This protein does not bind antibodies to bovine myelin basic protein, while trout myelin basic proteins in the same molecular weight range do. In addition, the protein does not bind concanavalin A or react with the periodic acid-Schiff reagent. The 13.6 kDa band represents about 1% of the total protein in saccular sensory epithelium, and may be a marker protein for the hair cell layer.
已检测到一种与虹鳟鱼（Salmo gairdnerii R.）球囊毛细胞层相关的蛋白质。通过一维和二维SDS聚丙烯酰胺凝胶电泳，该蛋白质的分子量和等电点估计分别为13.6 kDa和8.8 kDa。在脑、鳃、肝脏以及含有基底层、非感觉上皮和球囊神经的组分中，无法通过电泳检测到13.6 kDa的蛋白质。该蛋白质不与抗牛髓鞘碱性蛋白的抗体结合，而相同分子量范围内的鳟鱼髓鞘碱性蛋白则能结合。此外，该蛋白质不与伴刀豆球蛋白A结合，也不与高碘酸-希夫试剂反应。13.6 kDa的条带约占球囊感觉上皮总蛋白的1%，可能是毛细胞层的一种标记蛋白。
