Vasin A V, Petrova A V, Egorov V V, Plotnikova M A, Klotchenko S A, Karpenko M N, Kiselev O I
Research Institute of Influenza, 197376, St-Petersburg, Russia.
Peter the Great St-Petersburg Polytechnic University, 195251, St-Petersburg, Russia.
BMC Res Notes. 2016 May 20;9:279. doi: 10.1186/s13104-016-2083-6.
Influenza A virus (IAV) is a segmented negative-sense RNA virus that causes seasonal epidemics and periodic pandemics in humans. Two regions (nucleotide positions 82-148 and 497-564) in the positive-sense RNA of the NS segment fold into a multi-branch loop or hairpin structures.
We studied 25,384 NS segment positive-sense RNA unique sequences of human and non-human IAVs in order to predict secondary RNA structures of the 82-148 and 497-564 regions using RNAfold software, and determined their host- and lineage-specific distributions. Hairpins prevailed in avian and avian-origin human IAVs, including H1N1pdm1918 and H5N1. In human and swine IAV hairpins distribution varied between evolutionary lineages.
These results suggest a possible functional role for these RNA secondary structures and the need for experimental evaluation of these structures in the influenza life cycle.
甲型流感病毒（IAV）是一种分节段的负链RNA病毒，可在人类中引发季节性流行和周期性大流行。NS节段正链RNA中的两个区域（核苷酸位置82 - 148和497 - 564）折叠成多分支环或发夹结构。
我们研究了25384条人类和非人类IAV的NS节段正链RNA独特序列，以便使用RNAfold软件预测82 - 148和497 - 564区域的RNA二级结构，并确定它们在宿主和谱系中的特异性分布。发夹结构在禽类和禽源人类IAV中占主导，包括H1N1pdm1918和H5N1。在人类和猪IAV中，发夹结构的分布在进化谱系之间有所不同。
这些结果表明这些RNA二级结构可能具有功能作用，并且需要在流感生命周期中对这些结构进行实验评估。
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