蒙古牛和牦牛中牛病毒性腹泻病毒的分子检测与特性分析
Molecular detection and characterization of bovine viral diarrhea virus in Mongolian cattle and yaks.
作者信息
Ochirkhuu Nyamsuren, Konnai Satoru, Odbileg Raadan, Odzaya Battogtokh, Gansukh Shura, Murata Shiro, Ohashi Kazuhiko
机构信息
Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, 060-0818, Japan.
Laboratory of Virology, Institute of Veterinary Medicine, Mongolia University of Life Science, Khan-Uul district, Zaisan, 17042, Ulaanbaatar, Mongolia.
出版信息
Arch Virol. 2016 Aug;161(8):2279-83. doi: 10.1007/s00705-016-2890-z. Epub 2016 May 20.
Bovine viral diarrhea virus (BVDV) is classified into two species, namely, Bovine viral diarrhea virus 1 and Bovine viral diarrhea virus 2, and affects cattle worldwide, resulting in significant economic loss. The prevalence of BVDV-1 and BVDV-2 infections and its genotypes in Mongolian animals has not been studied. In this study, we surveyed BVDV infection in dairy cattle and yaks from Bornuur and Bulgan counties by RT-PCR, and the average infection rate in the sampling sites was 15.8 % and 20.0 %, respectively. In addition, molecular features of the 5'-UTR region of the BVDV genome in Mongolian cattle and yaks were identified as belonging to the subtypes BVDV-1a and BVDV-2a, respectively. Determining the prevalence, geographical distribution, and molecular diversity of BVDV-1 and BVDV-2 in various host species in Mongolia is important for further studies and process control programs.
牛病毒性腹泻病毒（BVDV）分为两个种，即牛病毒性腹泻病毒1型和牛病毒性腹泻病毒2型，在全球范围内影响牛群，造成重大经济损失。尚未对蒙古动物中BVDV - 1和BVDV - 2感染及其基因型的流行情况进行研究。在本研究中，我们通过逆转录聚合酶链反应（RT-PCR）对布尔干县和布尔根县的奶牛和牦牛进行了BVDV感染调查，采样点的平均感染率分别为15.8%和20.0%。此外，蒙古牛和牦牛BVDV基因组5'-非翻译区（5'-UTR）的分子特征分别被鉴定为属于BVDV - 1a和BVDV - 2a亚型。确定BVDV - 1和BVDV - 2在蒙古各种宿主物种中的流行情况、地理分布和分子多样性，对于进一步的研究和防控计划至关重要。
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