Gómez-Romero Ninnet, Basurto-Alcántara Francisco J, Verdugo-Rodríguez Antonio, Bauermann Fernando V, Ridpath Julia F
Vaccinology Laboratory (Gómez-Romero, Basurto-Alcántara), Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Molecular Microbiology Laboratory, Microbiology and Immunology Department (Verdugo-Rodríguez), Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Mexico City, Mexico.
J Vet Diagn Invest. 2017 May;29(3):362-365. doi: 10.1177/1040638717690187. Epub 2017 Apr 4.
Bovine viral diarrhea virus (BVDV) infects cattle populations worldwide, causing significant economic losses though its impact on animal health. Previous studies have reported the prevalence of BVDV species and subgenotypes in cattle from the United States and Canada. We investigated the genetic diversity of BVDV strains detected in bovine serum samples from 6 different Mexican regions. Sixty-two BVDV isolates from Mexico were genetically typed based on comparison of sequences from the 5' untranslated region (5'-UTR) of the viral genome. Phylogenetic reconstruction indicated that 60 of the samples belonged to the BVDV-1 genotype and 2 to the BVDV-2 genotype. Comparison of partial 5'-UTR sequences clustered 49 samples within BVDV-1c, 8 samples within BVDV-1a, 3 samples within BVDV-1b, and 2 samples clustered with the BVDV-2a subgenotypes. Our study, combined with information previously published on BVDV field strain diversity in the United States and Canada, benefits the development of effective detection assays, vaccines, and control programs for North America.
牛病毒性腹泻病毒（BVDV）感染全球的牛群，因其对动物健康的影响而造成重大经济损失。先前的研究报告了美国和加拿大牛群中BVDV物种和亚基因型的流行情况。我们调查了在墨西哥6个不同地区的牛血清样本中检测到的BVDV毒株的遗传多样性。基于病毒基因组5'非翻译区（5'-UTR）序列的比较，对来自墨西哥的62株BVDV分离株进行了基因分型。系统发育重建表明，60个样本属于BVDV-1基因型，2个样本属于BVDV-2基因型。部分5'-UTR序列的比较将49个样本聚类在BVDV-1c内，8个样本聚类在BVDV-1a内，3个样本聚类在BVDV-1b内，2个样本与BVDV-2a亚基因型聚类。我们的研究与先前发表的关于美国和加拿大BVDV田间毒株多样性的信息相结合，有助于北美地区开发有效的检测方法、疫苗和控制计划。
