Polácková A, Tlaskalová-Hogenová H, Spicák V, Polácková M, Mancal P
Cas Lek Cesk. 1989 Feb 17;128(8):232-7.
The authors describe a modification of the immunoenzymatic test suitable for estimation (sensitive dedection) of specific antibodies against pollen allergens of all classes of immunoglobulins, incl. specific IgE. When introducing the method, sera of experimental rabbits immunized with selected allergens were used. After elaboration of suitable conditions, the method was used to assess the presence of specific antibodies in sera of patients with the diagnosis of pollinosis. It was found that the most suitable concentration of pollen allergen combined with the solid phase for detection of specific IgE is 100 micrograms/ml, the optimal concentration of glutaraldehyde used to increase the amount of allergen combined with the solid phase by its polymerization and fixation is 0.25%. The use of the method does not call for special equipment and uses locally produced products. The method is useful for supplementing the diagnosis and its more accurate assessment under clinical conditions when biological tests cannot be used for diagnosis. The test makes monitoring during immunotherapy possible.
作者描述了一种免疫酶试验的改良方法，适用于检测（灵敏检测）针对所有免疫球蛋白类别（包括特异性IgE）的花粉过敏原的特异性抗体。在介绍该方法时，使用了用选定过敏原免疫的实验兔的血清。在确定了合适的条件后，该方法被用于评估诊断为花粉症患者血清中特异性抗体的存在情况。结果发现，用于检测特异性IgE的与固相结合的花粉过敏原的最适浓度为100微克/毫升，用于通过其聚合和固定增加与固相结合的过敏原量的戊二醛的最佳浓度为0.25%。该方法的使用不需要特殊设备，并且使用当地生产的产品。该方法在无法使用生物学试验进行诊断的临床条件下，有助于补充诊断并进行更准确的评估。该试验使免疫治疗期间的监测成为可能。
