Chen Yung-Ju, Wallig Matthew A, Jeffery Elizabeth H
Department of Food Science and Human Nutrition, University of Illinois, Urbana, Illinois, USA.
Department of Pathobiology, University of Illinois, Urbana, Illinois, USA.
Toxicol Pathol. 2016 Aug;44(6):913-6. doi: 10.1177/0192623316648803. Epub 2016 May 19.
Diethylnitrosamine (DEN) is a chemical broadly used in animal models as a hepatocarcinogen, reported to also cause pulmonary neoplasms in mice. The original objective was to evaluate the impact of a Western diet with or without 10% broccoli on DEN-induced on liver cancer. We administered DEN (45 mg/kg) intraperitoneally to young adult male B6C3F1 mice by 6 weekly injections and evaluated liver cancer 6 months after the DEN treatments. Here, we report unexpected primary tumorigenesis in nasal epithelium, independent of dietary treatment. More than 50% of DEN-treated B6C3F1 mice developed nasal neoplasm-related lesions, not reported previously in the literature. Only one of these neoplasms was visible externally prior to postmortem examination. Intraperitoneal DEN treatment used as a model for liver cancer can have a carcinogenic effect on the nasal epithelium in B6C3F1 mice, which should be carefully monitored in future liver cancer studies.
二乙基亚硝胺（DEN）是一种广泛用于动物模型的化学物质，作为一种肝癌致癌物，据报道它也会在小鼠中引发肺部肿瘤。最初的目的是评估含或不含10%西兰花的西方饮食对DEN诱导的肝癌的影响。我们通过每周6次腹腔注射，给年轻成年雄性B6C3F1小鼠腹腔注射DEN（45毫克/千克），并在DEN处理6个月后评估肝癌情况。在此，我们报告了在鼻上皮中意外出现的原发性肿瘤形成，这与饮食处理无关。超过50%接受DEN处理的B6C3F1小鼠出现了鼻肿瘤相关病变，这在以前的文献中未曾报道。在尸检前，这些肿瘤中只有一个在外部可见。用作肝癌模型的腹腔DEN处理可能会对B6C3F1小鼠的鼻上皮产生致癌作用，在未来的肝癌研究中应仔细监测这一点。
