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帕里牦牛(Bos grunniens)的全基因组测序及系统发育分析

Complete mitogenome sequencing and phylogenetic analysis of PaLi yak (Bos grunniens).

作者信息

Bao Pengjia, Guo Xian, Pei Jie, Liang Chunnian, Ding Xuezhi, Min Chu, Wang Hongbo, Wu Xiaoyun, Yan Ping

机构信息

a Gansu Agriculture University, Gansu Province , Lanzhou , China.

b Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Science , Lanzhou , China , and.

出版信息

Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Nov;27(6):4590-4591. doi: 10.3109/19401736.2015.1046128. Epub 2016 May 20.

Abstract

PaLi yak is a very important local breed in China; as a year-round grazing animal, it plays a very important role for the economic and native herdsmen. The PaLi yak complete mitochondrial DNA is sequenced in this study, the total length is 16,324 bp, containing 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a non-coding control region (D-loop region). The order and composition are similar to most of the other vertebrates. The base contents are: 33.72% A, 25.80% C, 13.21% G and 27.27% T; A + T (60.99%) was higher than G + C (39.01%). The phylogenetic relationships were analyzed using the complete mitogenome sequence, results showed that the genetic relationship between yak and cattle is distinct. These information provides useful data for further study on protection of genetic resources and the taxonomy of Bovinae.

摘要

帕里牦牛是中国非常重要的地方品种;作为一种全年放牧的家畜,它对当地经济和牧民起着非常重要的作用。本研究对帕里牦牛的线粒体DNA进行了全序列测定,全长16324bp,包含13个蛋白质编码基因、22个tRNA基因、2个rRNA基因和一个非编码控制区(D-loop区)。其排列顺序和组成与大多数其他脊椎动物相似。碱基含量分别为:A占33.72%、C占25.80%、G占13.21%、T占27.27%;A+T(60.99%)高于G+C(39.01%)。利用线粒体全基因组序列分析了系统发育关系,结果表明牦牛与牛之间的亲缘关系较远。这些信息为进一步研究牛亚科遗传资源保护和分类学提供了有用的数据。

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