Hu C Y, Novakofski J E, Mersmann H J
Department of Animal Sciences, University of Illinois, Urbana 61801.
Cell Biol Int Rep. 1989 Feb;13(2):171-80. doi: 10.1016/0309-1651(89)90063-5.
Isolated differentiated rat adipocytes embedded in a collagen matrix were maintained in culture for 0, 4 and 14 days. Assessment of viability by either anabolic glucose metabolism or catabolic lipolytic metabolism can be misleading; both types of metabolism should be measured to establish the status of cells. Cells maintained morphological integrity even when not viable as judged by anabolic glucose metabolism.
嵌入胶原基质中的分离的分化大鼠脂肪细胞在培养中维持0、4和14天。通过合成代谢性葡萄糖代谢或分解代谢性脂解代谢来评估细胞活力可能会产生误导；应该测量这两种代谢类型以确定细胞状态。即使根据合成代谢性葡萄糖代谢判断细胞无活力时，细胞仍保持形态完整性。
