Scalici Elodie, Bechoua Shaliha, Astruc Karine, Duvillard Laurence, Gautier Thomas, Drouineaud Véronique, Jimenez Clément, Hamamah Samir
ART-PGD Department, INSERM U1203, Arnaud de Villeneuve Hospital, CHU Montpellier, Montpellier, France.
University of Burgundy, UFR Sciences de Santé de Dijon, Dijon, France.
Reprod Biol Endocrinol. 2016 May 21;14(1):28. doi: 10.1186/s12958-016-0150-4.
Follicular fluid (FF) is an important micro-environment influencing oocyte growth, its development competence, and embryo viability. The FF content analysis allows to identify new relevant biomarkers, which could be predictive of in vitro fertilization (IVF) outcomes. Inside ovarian follicle, the amount of FF components from granulosa cells (GC) secretion, could be regulated by gonadotropins, which play a major role in follicle development.
This prospective study included 61 female undergoing IVF or Intra-cytoplasmic sperm injection (ICSI) procedure. Apolipoprotein B (APOB) concentrations in follicular fluid and APOB gene and protein expression in granulosa cells from reproductively aged women undergoing an in vitro fertilization program were measured. The statistical analyses were performed according to a quartile model based on the amount of APOB level found in FF.
Amounts of APOB were detected in human FF samples (mean ± SD: 244.6 ± 185.9 ng/ml). The odds of obtaining an oocyte in the follicle and a fertilized oocyte increased significantly when APOB level in FF was higher than 112 ng/ml [i.e., including in Quartile Q 2, Q3 and Q4] (p = 0.001; p < 0.001, respectively). The probabilities of obtaining an embryo and a top quality embryo on day 2, were significantly higher if APOB levels were within the ranges of 112 and 330 ng/ml (i.e. in Q2 and Q3) or 112 and 230 ng/ml (i.e. in Q2), respectively (p < 0.001; p = 0.047, respectively). In addition, our experiments in vitro indicated that APOB gene and protein expression, along with APOB content into culture were significantly under-expressed in GC upon stimulation with gonadotropins (follicular stimulating hormone: FSH and/or human chorionic gonadotropin: hCG).
We are reporting a positive and statistically significant associations between APOB and oocyte retrieval, oocyte fertilization, and embryo quality. Using an experimental study component, the authors report significant reduced APOB expression and content for luteinized granulosa cells cultured in the presence of gonadotropins.
卵泡液（FF）是影响卵母细胞生长、其发育能力和胚胎活力的重要微环境。卵泡液成分分析有助于识别新的相关生物标志物，这些标志物可预测体外受精（IVF）结果。在卵巢卵泡内，来自颗粒细胞（GC）分泌的卵泡液成分量可能受促性腺激素调节，促性腺激素在卵泡发育中起主要作用。
这项前瞻性研究纳入了61名接受IVF或卵胞浆内单精子注射（ICSI）手术的女性。测量了接受体外受精程序的育龄妇女卵泡液中载脂蛋白B（APOB）浓度以及颗粒细胞中APOB基因和蛋白表达。根据基于卵泡液中APOB水平的四分位数模型进行统计分析。
在人类卵泡液样本中检测到了APOB含量（平均值±标准差：244.6±185.9 ng/ml）。当卵泡液中APOB水平高于112 ng/ml时（即包括在四分位数Q2、Q3和Q4中），卵泡中获得卵母细胞和受精卵母细胞的几率显著增加（p = 0.001；p < 0.001，分别）。如果APOB水平在112至330 ng/ml范围内（即Q2和Q3）或112至230 ng/ml范围内（即Q2），在第2天获得胚胎和优质胚胎的概率分别显著更高（p < 0.001；p = 0.047，分别）。此外，我们的体外实验表明，在用促性腺激素（促卵泡激素：FSH和/或人绒毛膜促性腺激素：hCG）刺激后，颗粒细胞中APOB基因和蛋白表达以及培养物中的APOB含量显著下调。
我们报告了APOB与卵母细胞获取、卵母细胞受精和胚胎质量之间存在积极且具有统计学意义的关联。通过实验研究部分，作者报告在促性腺激素存在下培养的黄体化颗粒细胞中APOB表达和含量显著降低。
