Bliley Jacqueline M, Argenta Anne, Satish Latha, McLaughlin Meghan M, Dees Aaron, Tompkins-Rhoades Casey, Marra Kacey G, Rubin J Peter
Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA.
Department of Plastic Surgery, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, Pittsburgh, PA, USA.
Burns. 2016 Sep;42(6):1212-22. doi: 10.1016/j.burns.2015.12.007. Epub 2016 Jul 7.
Current treatment options for severe burn wounds are often insufficient in reconstructing skin and soft tissue defects. Adipose-derived stem cells (ASCs), a readily available source of multipotent stem cells, represent a promising therapy for the treatment of full-thickness burn wounds. Full-thickness burn wounds were created on the paraspinal region of athymic mice. A one-time, sub-eschar injection of 6.8×10(6) ASCs in PBS or PBS alone was administered at 24-h postoperatively. Time to healing was quantified using Image J analysis. At days 4, 7, 14, and 21, mice were sacrificed and tissues were excised for molecular and histological analysis. ASCs were able to survive in burn wounds as determined by the presence of PKH labeling and human PPARγ expression within the wounds. CD-31 staining demonstrated increased vascularity in ASC-treated wounds at POD 4 (p<0.05). Molecular studies showed enhanced adipogenesis, as well as type III and type I collagen deposition in the ASC treated group (p<0.05). An increase in the mRNA expression ratio of type III to type I collagen was also observed following ASC treatment (p<0.05). By enhancing vascularity, collagen deposition, and adipogenesis, ASCs show promise as an adjunctive therapy for the current treatment of full thickness burn wounds.
目前针对严重烧伤创面的治疗方案在重建皮肤和软组织缺损方面往往并不充分。脂肪来源干细胞（ASCs）作为一种易于获取的多能干细胞来源，是治疗全层烧伤创面的一种有前景的疗法。在无胸腺小鼠的脊柱旁区域制造全层烧伤创面。术后24小时，在创缘下一次性注射6.8×10⁶个处于磷酸盐缓冲液（PBS）中的脂肪来源干细胞或仅注射PBS。使用Image J分析对愈合时间进行量化。在第4天、第7天、第14天和第21天，处死小鼠并切除组织进行分子和组织学分析。通过伤口内PKH标记和人PPARγ表达的存在确定脂肪来源干细胞能够在烧伤创面存活。CD - 31染色显示在术后第4天（POD 4），接受脂肪来源干细胞治疗的伤口血管生成增加（p<0.05）。分子研究表明，脂肪来源干细胞治疗组的脂肪生成增强，同时III型和I型胶原蛋白沉积增加（p<0.05）。脂肪来源干细胞治疗后还观察到III型与I型胶原蛋白mRNA表达比值增加（p<0.05）。通过增强血管生成、胶原蛋白沉积和脂肪生成，脂肪来源干细胞有望成为当前全层烧伤创面治疗的辅助疗法。
