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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Tomić S, Sesartić L, Tomasić J

Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia.

Comp Biochem Physiol B. 1989;92(4):681-4. doi: 10.1016/0305-0491(89)90249-6.

14C-labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a substrate for esterases from rabbit, guinea pig, mouse, donkey, pig, horse, sheep and human sera. 2. Stepwise de-esterification of the diester substrate 1 occurred with rabbit, guinea pig and mouse serum. Data on time-course experiments and kinetic data are reported. 3. The use of donkey, pig, horse, sheep and human serum led to the migration of the 2-O-pivaloyl group in substrate 1 to the position 4- in the sugar molecule, followed by stepwise de-esterifications of both 1 and the newly formed methyl 4,6-di-O-pivaloyl-alpha-D-glucopyranoside (4). A report is given on the time-course experiments.

用14C标记的2,6-二-O-新戊酰基-α-D-吡喃葡萄糖苷甲酯（1）作为兔、豚鼠、小鼠、驴、猪、马、羊和人血清中酯酶的底物。2. 二酯底物1在兔、豚鼠和小鼠血清中发生逐步脱酯反应。报告了时间进程实验数据和动力学数据。3. 使用驴、猪、马、羊和人血清时，底物1中的2-O-新戊酰基迁移至糖分子的4-位，随后1和新形成的4,6-二-O-新戊酰基-α-D-吡喃葡萄糖苷甲酯（4）都发生逐步脱酯反应。给出了时间进程实验报告。

Tomić S, Sesartić L, Tomasić J

Department of Radioimmunology, Institute of Immunology, Zagreb, Yugoslavia.

Comp Biochem Physiol B. 1989;92(4):681-4. doi: 10.1016/0305-0491(89)90249-6.

14C-labelled methyl 2,6-di-O-pivaloyl-alpha-D-glucopyranoside (1) was used as a substrate for esterases from rabbit, guinea pig, mouse, donkey, pig, horse, sheep and human sera. 2. Stepwise de-esterification of the diester substrate 1 occurred with rabbit, guinea pig and mouse serum. Data on time-course experiments and kinetic data are reported. 3. The use of donkey, pig, horse, sheep and human serum led to the migration of the 2-O-pivaloyl group in substrate 1 to the position 4- in the sugar molecule, followed by stepwise de-esterifications of both 1 and the newly formed methyl 4,6-di-O-pivaloyl-alpha-D-glucopyranoside (4). A report is given on the time-course experiments.

用14C标记的2,6-二-O-新戊酰基-α-D-吡喃葡萄糖苷甲酯（1）作为兔、豚鼠、小鼠、驴、猪、马、羊和人血清中酯酶的底物。2. 二酯底物1在兔、豚鼠和小鼠血清中发生逐步脱酯反应。报告了时间进程实验数据和动力学数据。3. 使用驴、猪、马、羊和人血清时，底物1中的2-O-新戊酰基迁移至糖分子的4-位，随后1和新形成的4,6-二-O-新戊酰基-α-D-吡喃葡萄糖苷甲酯（4）都发生逐步脱酯反应。给出了时间进程实验报告。