Yan Zhangming, Liu Ke, Xiang Shunian, Sun Zhirong
IEEE/ACM Trans Comput Biol Bioinform. 2017 May-Jun;14(3):746-748. doi: 10.1109/TCBB.2016.2568178. Epub 2016 May 16.
Since the development of new technologies such as RIP-Seq and mA-seq, peak calling has become an important step in transcriptomic sequencing data analysis. However, many of the reported genomic coordinates of transcriptomic peaks are incorrect owing to negligence of the introns. There is currently a lack of a convenient tool to address this problem. Here, we present txCoords, a novel and easy-to-use web application for transcriptomic peak re-mapping. txCoords can be used to correct the incorrectly reported transcriptomic peaks and retrieve the true sequences. It also supports visualization of the re-mapped peaks in a schematic figure or from the UCSC Genome Browser. Our web server is freely available at http://www.bioinfo.tsinghua.edu.cn/txCoords.
自从RIP-Seq和mA-seq等新技术出现以来,峰检测已成为转录组测序数据分析中的重要步骤。然而,由于内含子被忽视,许多报道的转录组峰的基因组坐标是错误的。目前缺乏解决这一问题的便捷工具。在此,我们展示了txCoords,这是一种用于转录组峰重新映射的新颖且易于使用的网络应用程序。txCoords可用于校正错误报道的转录组峰并检索真实序列。它还支持在示意图中或从UCSC基因组浏览器可视化重新映射的峰。我们的网络服务器可在http://www.bioinfo.tsinghua.edu.cn/txCoords免费获取。