[KIF18A在胃癌中的表达及其与预后的关系]

[Expression of KIF18A in gastric cancer and its association with prognosis].

作者信息

Wang Li, Yang Song, Sun Ruochuan, Lu Mingdian, Wu Youliang, Li Yongxiang

机构信息

Department of General Surgery, the First Affiliated Hosptial of Anhui Medical University, Hefei 230022, China.

出版信息

Zhonghua Wei Chang Wai Ke Za Zhi. 2016 May;19(5):585-9.

PMID:27215532

Abstract

OBJECTIVE

To explore the expression of KIF18A gene protein in gastric cancer tissues and its association with the prognosis of patients.

METHODS

Twenty fresh paired gastric cancer specimens and adjacent normal mucosa(at least 5 cm from the edge of tumor) from 20 gastric cancer patients undergoing operation in Department of General Surgery at the First Affiliated Hosptial of Anhui Medical University between March 2015 and July 2015 were collected. Real-time PCR was used to examine KIF18A mRNA expression in above specimens. Meanwhile, paraffin embedded cancer tissue samples from 129 gastric cancer patients undergoing operation and 23 samples of randomly selected normal gastric tissue(adjacent non-cancer tissue) were collected to establish the microarray. Immunohistochemistry method was applied to detect the KIF18A protein expression in the microarray after confirmation by pathologists. Association of KIF18A expression with clinicopathological features in gastric cancer patients was evaluated. Cox proportional hazard model was used to identify prognostic risk factors.

RESULTS

Among 20 fresh paired gastric cancer specimens, mRNA expression of KIF18A in 16 specimens was obviously lower than that in adjacent normal tissues. The positive rate of KIF18A protein expression in gastric cancer tissues was significantly lower than that in normal gastric tissues in microarray[45.0%(58/129) vs. 69.6%(16/23), P=0.041]. KIF18A protein expression was significantly associated with invasion depth (P=0.008) and TNM staging (P=0.032). The median overall survival of all the 129 patients was 44.0(95% CI: 39.78-49.24) months. The three-year survival rates of patients with high and low KIF18A expression were 67.2% and 36.6% respectively(P=0.020). Cox regression analysis showed that KIF18A expression was an independent protective factor of the prognosis of gastric cancer patients (HR=0.570, 95% CI:0.335 to 0.970).

CONCLUSIONS

KIF18A expression is down-regulated in gastric cancer tissue, which may play a critical role in gastric cancer carcinogenesis. Lower expression of KIF18A is associated with poor prognosis of gastric cancer patients. KIF18A may be a potential prognostic marker of gastric cancer.

摘要

目的

探讨KIF18A基因蛋白在胃癌组织中的表达及其与患者预后的关系。

方法

收集2015年3月至2015年7月在安徽医科大学第一附属医院普外科接受手术的20例胃癌患者的20对新鲜配对胃癌标本及癌旁正常黏膜（距肿瘤边缘至少5 cm）。采用实时荧光定量PCR检测上述标本中KIF18A mRNA的表达。同时，收集129例接受手术的胃癌患者的石蜡包埋癌组织样本及23例随机选取的正常胃组织（癌旁非癌组织）样本制作芯片。经病理学家确认后，采用免疫组织化学方法检测芯片中KIF18A蛋白的表达。评估KIF18A表达与胃癌患者临床病理特征的相关性。采用Cox比例风险模型确定预后危险因素。

结果

在20对新鲜配对胃癌标本中，16例标本中KIF18A的mRNA表达明显低于癌旁正常组织。芯片中胃癌组织KIF18A蛋白表达阳性率显著低于正常胃组织[45.0%（58/129）对69.6%（16/23），P = 0.041]。KIF18A蛋白表达与浸润深度（P = 0.008）和TNM分期（P = 0.032）显著相关。129例患者的中位总生存期为44.0（95%CI：39.78 - 49.24）个月。KIF18A表达高低患者的三年生存率分别为67.2%和36.6%（P = 0.020）。Cox回归分析显示，KIF18A表达是胃癌患者预后的独立保护因素（HR = 0.570，95%CI：0.335至0.970）。