Wu Yaqin, Wang Anpeng, Zhu Biqing, Huang Jian, Lu Emei, Xu Hanzi, Xia Wenjie, Dong Gaochao, Jiang Feng, Xu Lin
Jiangsu Key Laboratory of Molecular and Translational Cancer Research, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Department of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Onco Targets Ther. 2018 Mar 28;11:1707-1720. doi: 10.2147/OTT.S157440. eCollection 2018.
KIF18B was identified as a potential oncogene by analysis of The Cancer Genome Atlas database.
We assessed KIF18B expression and explored its clinical significance in cervical cancer tissues. We have also evaluated the effects of KIF18B on cervical cancer cell proliferation, migration, and invasion both in vitro and in vivo.
Our results show that KIF18B is overexpressed in cervical cancer tissues and is associated with a large primary tumor size, an advanced FIGO stage, and an advanced tumor grade. Knockdown of KIF18B induces cell cycle G1-phase arrest and inhibits the proliferation, migration, and invasion of cervical cancer cells, whereas its overexpression promotes proliferation, migration, and invasion in these cells. Moreover, silencing of KIF18B reduces expression of CyclinD1, β-catenin, C-myc, and p-GSK3β expression.
These data suggest that KIF18B can serve as a novel oncogene that promotes the tumorigenicity of cervical cancer cells by activating Wnt/β-catenin signaling pathway.
通过对癌症基因组图谱数据库的分析，KIF18B被鉴定为一种潜在的癌基因。
我们评估了KIF18B在宫颈癌组织中的表达，并探讨了其临床意义。我们还在体外和体内评估了KIF18B对宫颈癌细胞增殖、迁移和侵袭的影响。
我们的结果表明，KIF18B在宫颈癌组织中过表达，并且与较大的原发肿瘤大小、较高的国际妇产科联盟（FIGO）分期和较高的肿瘤分级相关。敲低KIF18B可诱导细胞周期G1期阻滞，并抑制宫颈癌细胞的增殖、迁移和侵袭，而其过表达则促进这些细胞的增殖、迁移和侵袭。此外，沉默KIF18B可降低细胞周期蛋白D1、β-连环蛋白、C- myc和磷酸化糖原合成酶激酶3β（p-GSK3β）的表达。
这些数据表明，KIF18B可作为一种新型癌基因，通过激活Wnt/β-连环蛋白信号通路促进宫颈癌细胞的致瘤性。
