Effect of DNA supercoiling on in vitro transcription from the adenovirus early region 4.
作者信息
Handa H, Watanabe H, Suzuki Y, Hirose S
机构信息
Department of Bacteriology, University of Tokyo Faculty of Medicine, Japan.
出版信息
FEBS Lett. 1989 May 22;249(1):17-20. doi: 10.1016/0014-5793(89)80006-7.
PMID:2721686
Abstract
The effect of DNA supercoiling on in vitro transcription from the early region 4 (E4) promoter of adenovirus type 5 (Ad5) has been investigated by using a cell-free transcription system that maintains supercoiled DNA templates. The supercoiled DNAs yield several-fold higher levels of E4 transcripts and more faithfully reflect the regulation of in vivo transcription than the linear DNAs.
摘要
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