Suriyachadkun Chanwit, Ngaemthao Wipaporn, Chunhametha Suwanee
Biotec Culture Collection, Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), 113 Thailand Science Park, Phaholyothin Road, Khlong Nueng, Khlong Luang, 12120 Pathum Thani, Thailand.
Int J Syst Evol Microbiol. 2016 Aug;66(8):3224-3229. doi: 10.1099/ijsem.0.001178. Epub 2016 May 23.
A novel actinomycete strain, A-T 11038T, was isolated from bamboo rhizospheric soil collected in Thailand. Based on a polyphasic approach, the novel strain was characterized as a member of the genus Planomonospora, which developed cylindrical to clavate sporangia containing a single motile spore on aerial mycelium. The 16S rRNA gene sequence and phylogenetic analysis indicated that strain A-T 11038T was closely related to Planomonospora sphaerica JCM 9374T (98.82 %), P.lanomonospora parontospora subsp. parontospora NBRC 13880T and P.parontospora subsp. antibiotica JCM 3094T (98.54 %), Planomonospora alba JCM 9373T (98.41 %) and Planomonospora venezuelensis JCM 3167T (97.51 %). The DNA-DNA relatedness values that distinguished strain A-T 11038T from the most closely related species were below 45 %. The novel strain contained meso-diaminopimelic acid in cell-wall hydrolysates, and rhamnose, ribose, madurose and glucose in whole-cell hydrolysates. The predominant menaquinone was MK-9(H2). The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylinositol and aminophosphoglycolipids. The predominant cellular fatty acids were unsaturated fatty acids C17 : 1 and C16 : 1 and saturated fatty acid C16 : 0. The G+C content of the genomic DNA was 73.5 mol%. Following the evidence obtained using a polyphasic approach, the novel strain is proposed as a representative of a novel species to be named Planomonospora corallina sp. nov. The type strain is A-T 11038T (=BCC 67829T=TBRC 4489T=NBRC 110609T).
从泰国采集的竹根际土壤中分离出一株新型放线菌菌株A-T 11038T。基于多相分类法，该新型菌株被鉴定为扁平单孢菌属的成员，其在气生菌丝体上形成圆柱形至棒状的孢子囊，内含单个具鞭毛的孢子。16S rRNA基因序列和系统发育分析表明，菌株A-T 11038T与球形扁平单孢菌JCM 9374T（98.82%）、类亲本扁平单孢菌亲本亚种NBRC 13880T和类亲本扁平单孢菌抗生亚种JCM 3094T（98.54%）、白色扁平单孢菌JCM 9373T（98.41%）以及委内瑞拉链孢单孢菌JCM 3167T（97.51%）密切相关。将菌株A-T 11038T与最密切相关物种区分开来的DNA-DNA杂交值低于45%。该新型菌株细胞壁水解物中含有内消旋二氨基庚二酸，全细胞水解物中含有鼠李糖、核糖、马杜拉糖和葡萄糖。主要的甲基萘醌为MK-9(H2)。诊断性磷脂为双磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇甘露糖苷、磷脂酰肌醇和氨基磷脂糖脂。主要的细胞脂肪酸为不饱和脂肪酸C17:1和C16:1以及饱和脂肪酸C16:0。基因组DNA的G+C含量为73.5 mol%。根据多相分类法获得的证据，该新型菌株被提议作为一个新物种的代表，命名为珊瑚扁平单孢菌（Planomonospora corallina sp. nov.）。模式菌株为A-T 11038T（=BCC 67829T=TBRC 4489T=NBRC 110609T）。
