Granberg F, Bálint Á, Belák S
Rev Sci Tech. 2016 Apr;35(1):25-42. doi: 10.20506/rst.35.1.2415.
Next-generation sequencing (NGS), also referred to as deep, high-throughput or massively parallel sequencing, is a powerful new tool that can be used for the complex diagnosis and intensive monitoring of infectious disease in veterinary medicine. NGS technologies are also being increasingly used to study the aetiology, genomics, evolution and epidemiology of infectious disease, as well as host-pathogen interactions and other aspects of infection biology. This review briefly summarises recent progress and achievements in this field by first introducing a range of novel techniques and then presenting examples of NGS applications in veterinary infection biology. Various work steps and processes for sampling and sample preparation, sequence analysis and comparative genomics, and improving the accuracy of genomic prediction are discussed, as are bioinformatics requirements. Examples of sequencing-based applications and comparative genomics in veterinary medicine are then provided. This review is based on novel references selected from the literature and on experiences of the World Organisation for Animal Health (OIE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Uppsala, Sweden.
下一代测序（NGS），也被称为深度测序、高通量测序或大规模平行测序，是一种强大的新工具，可用于兽医学中传染病的复杂诊断和密集监测。NGS技术也越来越多地用于研究传染病的病因、基因组学、进化和流行病学，以及宿主-病原体相互作用和感染生物学的其他方面。本综述首先介绍一系列新技术，然后展示NGS在兽医感染生物学中的应用实例，简要总结该领域的最新进展和成就。讨论了采样和样品制备、序列分析和比较基因组学以及提高基因组预测准确性的各种工作步骤和流程，以及生物信息学要求。随后提供了兽医学中基于测序的应用和比较基因组学的实例。本综述基于从文献中选取的新参考文献以及瑞典乌普萨拉世界动物卫生组织（OIE）兽医学传染病生物技术诊断合作中心的经验。
