Tekel J, Farkas P, Kovác M
Food Research Institute, Bratislava, Czechoslovakia.
Food Addit Contam. 1989 Jul-Sep;6(3):377-81. doi: 10.1080/02652038909373793.
A method for the determination of acrylamide traces as a residue of anti-incrustation agents in sugar was developed. Acrylamide was extracted into ethyl acetate after derivatization by bromination to 2,3-dibromopropionamide (2,3-DBPA). The extract was cleaned up on a silica gel column and analysed by gas-liquid chromatography with an alkali flame-ionization detector (AFID). A glass capillary column with OV-1 stationary phase was used for this analysis. The recovery of the method determined in model experiments was 70.0% and 77.1% at 100 micrograms kg-1 and 20 micrograms kg-1, respectively. The limit of detection of acrylamide in sugar is 1-10 micrograms kg-1. Parameters of the described method are compared with other published methods.
开发了一种测定糖中作为防垢剂残留的痕量丙烯酰胺的方法。通过溴化将丙烯酰胺衍生化为2,3-二溴丙酰胺（2,3-DBPA）后，将其萃取到乙酸乙酯中。萃取液在硅胶柱上进行净化，并用碱火焰离子化检测器（AFID）进行气液色谱分析。采用带有OV-1固定相的玻璃毛细管柱进行该分析。在模型实验中，该方法在100微克/千克和20微克/千克时的回收率分别为70.0%和77.1%。糖中丙烯酰胺的检测限为1 - 10微克/千克。将所述方法的参数与其他已发表的方法进行了比较。
