采用电子捕获气相色谱法测定神经组织匀浆中的丙烯酰胺。

Determination of acrylamide in nerve tissue homogenates by electron-capture gas chromatography.

作者信息

Poole C F, Sye W F, Zlatkis A, Spencer P S

出版信息

J Chromatogr. 1981 Nov 6;217:239-45. doi: 10.1016/s0021-9673(00)88078-4.

PMID:7320111

Abstract

Acrylamide in biological samples can be determined by gas chromatography with electron-capture detection after conversion to its 2,3-dibromopropionamide derivative. The derivatization is carried out in aqueous solution, plasma or tissue homogenates by ionic bromination and the reaction conditions and sample clean-up are described. The detection limit corresponds to 9.5.10(-12)g of acrylamide on column or 8.4.10(-9)g in the final biological extract (0.5 ml). The overall recovery of acrylamide spiked samples at the nanogram level exceeds 80%. It was found that the accumulation of free acrylamide in the sciatic nerve distal region of rats intoxicated with acrylamide was less than 2-8 ppm.

摘要

生物样品中的丙烯酰胺在转化为其2,3 - 二溴丙酰胺衍生物后，可通过带有电子捕获检测的气相色谱法进行测定。衍生化反应在水溶液、血浆或组织匀浆中通过离子溴化进行，并描述了反应条件和样品净化方法。检测限相当于柱上9.5×10⁻¹²克丙烯酰胺或最终生物提取物（0.5毫升）中8.4×10⁻⁹克丙烯酰胺。纳克级加标丙烯酰胺样品的总回收率超过80%。研究发现，用丙烯酰胺中毒的大鼠坐骨神经远端区域中游离丙烯酰胺的积累量小于2 - 8 ppm。