Mofarrah Mohsen, Ziaee Shayan, Pilehvar-Soltanahmadi Yones, Zarghami Faraz, Boroumand Mohammadali, Zarghami Nosratollah
aDepartment of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz Departments of bMolecular Pathology cPathology and Laboratory Medicine, Tehran Heart Center, Tehran University of Medical Sciences, Tehran, Iran.
Coron Artery Dis. 2016 Sep;27(6):490-6. doi: 10.1097/MCA.0000000000000386.
Recently, several genes have been introduced as potential genetic markers for diabetes mellitus and coronary artery diseases (CAD).
In this case-control study, the associations of rs2241766 T/G of ADIPOQ, rs9289231 T/G of KALRN, and rs9939609 A/T of FTO polymorphisms with genetic susceptibility to CAD in type 2 diabetic (T2D) patients were investigated. A total of 224 T2D patients undergoing coronary angiography were randomly recruited into the study. Of the total diabetic patients, 152 were also diagnosed with CAD, whereas the rest were control participants. Genotyping of single-nucleotide polymorphisms was performed by high-resolution melting analysis.
Genotype analysis showed that the minor allele (G) frequency of rs2241766 ADIPOQ was statistically significant in the CAD group compared with the control group [odds ratio (OR), 2.779; 95% confidence interval (CI), 1.403-5.504; P=0.003]. Also, it was found that the minor allele (G) frequency of rs9289231 KALRN was significantly associated with the risk of CAD (OR, 2.098; 95% CI, 1.096-4.017; P=0.025). In addition, no significant association was observed between the minor allele (A) of the FTO rs9939609 polymorphism and CAD (OR, 1.088; 95% CI, 0.578-2.015; P=0.788). It is speculated that the GG genotype and the G allele of the rs9289231 polymorphism of KALRN and the rs224766 polymorphism of ADIPOQ genes may be considered genetic risk factors for CAD in T2D patients and genetic variations of these genes may play a major role in the process of these disorders.
Our case-control study in the Iranian population suggested a possible association between the mentioned single-nucleotide polymorphisms and CAD in T2D patients. However, further replication studies and comprehensive meta-analyses are required.
最近，有几个基因已被提出作为糖尿病和冠状动脉疾病（CAD）的潜在遗传标记。
在这项病例对照研究中，调查了脂联素（ADIPOQ）基因的rs2241766 T/G、Kalirin（KALRN）基因的rs9289231 T/G以及肥胖相关基因（FTO）多态性的rs9939609 A/T与2型糖尿病（T2D）患者CAD遗传易感性之间的关联。总共224名接受冠状动脉造影的T2D患者被随机纳入研究。在所有糖尿病患者中，152人也被诊断患有CAD，其余为对照参与者。通过高分辨率熔解分析进行单核苷酸多态性基因分型。
基因型分析表明，与对照组相比，CAD组中脂联素基因rs2241766的次要等位基因（G）频率具有统计学意义[比值比（OR），2.779；95%置信区间（CI），1.403 - 5.504；P = 0.003]。此外，发现Kalirin基因rs9289231的次要等位基因（G）频率与CAD风险显著相关（OR，2.098；95% CI，1.096 - 4.017；P = 0.025）。此外，未观察到FTO基因rs9939609多态性的次要等位基因（A）与CAD之间存在显著关联（OR，1.088；95% CI，0.578 - 2.015；P = 0.788）。据推测，Kalirin基因rs9289231多态性和脂联素基因rs224766多态性的GG基因型和G等位基因可能被视为T2D患者CAD 的遗传危险因素，这些基因的遗传变异可能在这些疾病的发生过程中起主要作用。
我们在伊朗人群中的病例对照研究表明，上述单核苷酸多态性与T2D患者的CAD之间可能存在关联。然而，需要进一步的重复研究和全面的荟萃分析。
