Velasquez M T, Kimmel P L, Michaelis O E, Carswell N, Abraham A, Bosch J P
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.
Diabetes. 1989 Jun;38(6):679-85. doi: 10.2337/diab.38.6.679.
The SHR/N corpulent (cp) rat is a genetically obese rat that develops hyperglycemia, hyperinsulinemia, and proteinuria. This study was designed to evaluate the effects of high carbohydrate (CHO) intake on renal function and structure in this animal model and to determine whether the renal effects are related to the type of CHO ingested. Two groups of 5-wk-old obese male SHR/N-cp rats and lean male littermates were fed diets containing 54% CHO in the form of sucrose or starch. After 12 wk, renal function parameters, including creatinine clearance, urinary glucose excretion, and urinary protein excretion, were measured. Renal morphology was evaluated by semiquantitative light and electron microscopy. On either diet, obese rats had significantly higher urinary glucose and protein excretions than their lean littermates. Mean creatinine clearance (ml/min) in obese rats did not differ significantly from values observed in lean rats. When corrected for body weight, creatinine clearance (ml.min-1.kg-1) tended to be lower in obese than in lean rats, but the difference was significant (P less than .02) only for obese and lean sucrose-fed animals. Obese rats fed sucrose compared with their obese counterparts fed starch had higher body weight (+8%, P less than .05), glucose excretion (+63%, P less than .02), and protein excretion (+242%, P less than .005). In obese rats, protein excretion correlated with glucose excretion (r = .71, P less than .01). Glomerular lesions consisting of mesangial expansion and intercapillary nodules were found in obese but not in lean rats. Moreover, obese rats fed sucrose had a significantly greater number of involved glomeruli than obese rats fed starch.(ABSTRACT TRUNCATED AT 250 WORDS)
SHR/N肥胖（cp）大鼠是一种遗传性肥胖大鼠，会出现高血糖、高胰岛素血症和蛋白尿。本研究旨在评估高碳水化合物（CHO）摄入量对该动物模型肾功能和结构的影响，并确定肾脏效应是否与摄入的CHO类型有关。两组5周龄的肥胖雄性SHR/N-cp大鼠和瘦雄性同窝仔鼠分别喂食含有54%以蔗糖或淀粉形式存在的CHO的日粮。12周后，测量肾功能参数，包括肌酐清除率、尿葡萄糖排泄量和尿蛋白排泄量。通过半定量光镜和电镜评估肾脏形态。无论喂食哪种日粮，肥胖大鼠的尿葡萄糖和蛋白排泄量均显著高于其瘦同窝仔鼠。肥胖大鼠的平均肌酐清除率（ml/min）与瘦大鼠观察到的值无显著差异。校正体重后，肥胖大鼠的肌酐清除率（ml·min-1·kg-1）倾向于低于瘦大鼠，但仅肥胖和瘦蔗糖喂养动物之间的差异具有统计学意义（P<0.02）。与喂食淀粉的肥胖大鼠相比，喂食蔗糖的肥胖大鼠体重更高（+8%，P<0.05），葡萄糖排泄量更高（+63%，P<0.02），蛋白排泄量更高（+242%，P<0.005）。在肥胖大鼠中，蛋白排泄量与葡萄糖排泄量相关（r = 0.71，P<0.01）。在肥胖大鼠而非瘦大鼠中发现了由系膜扩张和毛细血管间结节组成的肾小球病变。此外，喂食蔗糖的肥胖大鼠受累肾小球数量明显多于喂食淀粉的肥胖大鼠。（摘要截断于250字）
