Babaheydari Samad Bahrami, Keyvanshokooh Saeed, Dorafshan Salar, Johari Seyed Ali
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran.
Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Khouzestan, Iran.
Comp Biochem Physiol Part D Genomics Proteomics. 2016 Sep;19:1-7. doi: 10.1016/j.cbd.2016.05.001. Epub 2016 May 14.
A proteomic screening approach was employed to achieve a better understanding of the changes that occur in protein expression patterns associated with skeletal deformities in both diploid and triploid rainbow trout larvae. Triploidy was induced through the application of heat shock of 28°C for 10min to eggs 10-min post fertilization in an aquarium equipped with a heater. Percentage of skeletal deformity in heat-shocked larvae (2.88±0.30, mean±S.E.) was significantly (P<0.05) greater than that of the diploids (0.55±0.24). At five days after hatching, proteins of normal and deformed specimens of deyolked larvae were subjected to proteomic analysis using two-dimensional electrophoresis and mass spectrometry. Among the identified protein spots from diploids, creatine kinase was found to be increased in larvae with skeletal deformities, while apolipoprotein A-I-2, apolipoprotein A-II and calmodulin were found to be decreased in deformed fish. Among the five protein spots that were identified in heat-shocked fish, apolipoprotein A-I-2, apolipoprotein A-II, parvalbumin, myosin light chain 1-1 and nucleoside diphosphate kinase were found to be decreased in deformed larvae. The identification of nine protein spots showing altered expression in deformed fish allows us to reach a preliminary view of the molecular mechanisms that are involved in the development of skeletal malformations in diploid and triploid fish.
采用蛋白质组学筛选方法，以更好地了解二倍体和三倍体虹鳟鱼幼体骨骼畸形相关蛋白质表达模式的变化。在配备加热器的水族箱中，受精后10分钟的鱼卵经28°C热休克处理10分钟来诱导三倍体。热休克幼体的骨骼畸形百分比（2.88±0.30，平均值±标准误）显著（P<0.05）高于二倍体（0.55±0.24）。孵化后五天，对去卵黄幼体正常和畸形标本的蛋白质进行二维电泳和质谱分析。在二倍体中鉴定出的蛋白质斑点中，发现骨骼畸形的幼体中肌酸激酶增加，而畸形鱼中载脂蛋白A-I-2、载脂蛋白A-II和钙调蛋白减少。在热休克鱼中鉴定出的五个蛋白质斑点中，发现畸形幼体中载脂蛋白A-I-2、载脂蛋白A-II、小清蛋白、肌球蛋白轻链1-1和核苷二磷酸激酶减少。对畸形鱼中九个表达改变的蛋白质斑点的鉴定，使我们能够初步了解二倍体和三倍体鱼类骨骼畸形发育所涉及的分子机制。
