Lohmann W, Strobel J, Jacobi K W, Schmehl W, Bernhardt P, Wickert H
Fortschr Ophthalmol. 1989;86(1):23-5.
For various reasons, it is desirable to have an objective cataract classification system that does not depend on either the opinion of the physician or the patient. Since both highly molecular protein aggregates and chromophores are formed in cataractous lenses, which fluoresce under suitable stimulation, we have developed a fluorescence apparatus that illuminates the lens with five monochromatic wavelengths between 350 and 500 nm in situ. The fluorescence spectrum is recorded up to 100 ms with an optical multi-channel analyzer. This fluorescence method is non-invasive and does not harm the eye; it also uses the native fluorophore. The set of fluorescence spectra obtained by this method describes the condition of the cataractous lens both quantitatively and qualitatively. Because of the sensitivity of the method, it can also be used to assess the disease objectively in the early stages and to test and determine the effectiveness of anti-cataract drugs. Another use was discovered by using the fluorescence measurements for the diagnosis of malignant melanomas. With this equipment, melanomas in the eye and the surrounding area can be diagnosed both rapidly and precisely.
出于各种原因，需要有一个不依赖于医生或患者意见的客观白内障分类系统。由于在白内障晶状体中会形成高分子蛋白质聚集体和发色团，它们在适当刺激下会发出荧光，我们开发了一种荧光装置，可在原位用350至500纳米之间的五个单色波长照射晶状体。用光学多通道分析仪记录长达100毫秒的荧光光谱。这种荧光方法是非侵入性的，不会伤害眼睛；它还使用天然荧光团。通过这种方法获得的荧光光谱集从定量和定性两方面描述了白内障晶状体的状况。由于该方法的灵敏度，它还可用于在早期阶段客观评估疾病，并测试和确定抗白内障药物的有效性。通过使用荧光测量来诊断恶性黑色素瘤还发现了另一种用途。利用该设备，可以快速、精确地诊断眼睛及周围区域的黑色素瘤。
