Koçak Hasan Emre, Taşkın Ümit, Aydın Salih, Oktay Mehmet Faruk, Altınay Serdar, Çelik Duygu Sultan, Yücebaş Kadir, Altaş Bengül
Department of Otorhinolaryngology, Bakırköy Training and Research Hospital, Istanbul, Turkey.
Department of Otorhinolaryngology, Bağcılar Training and Research Hospital, Istanbul, Turkey.
Eur Arch Otorhinolaryngol. 2016 Dec;273(12):4153-4159. doi: 10.1007/s00405-016-4104-4. Epub 2016 May 24.
The aim of this study is to investigate the effect of rectal ozone and intratympanic ozone therapy on cisplatin-induced ototoxicity in rats. Eighteen female Wistar albino rats were included in our study. External auditory canal and tympanic membrane examinations were normal in all rats. The rats were randomly divided into three groups. Initially, all the rats were tested with distortion product otoacoustic emissions (DPOAE), and emissions were measured normally. All rats were injected with 5-mg/kg/day cisplatin for 3 days intraperitoneally. Ototoxicy had developed in all rats, as confirmed with DPOAE after 1 week. Rectal and intratympanic ozone therapy group was Group 1. No treatment was administered for the rats in Group 2 as the control group. The rats in Group 3 were treated with rectal ozone. All the rats were tested with DPOAE under general anesthesia, and all were sacrificed for pathological examination 1 week after ozone administration. Their cochleas were removed. The outer hair cell damage and stria vascularis damage were examined. In the statistical analysis conducted, a statistically significant difference between Group 1 and Group 2 was observed in all frequencies according to the DPOAE test. In addition, between Group 2 and Group 3, a statistically significant difference was observed in the DPOAE test. However, a statistically significant difference was not observed between Group 1 and Group 3 according to the DPOAE test. According to histopathological scoring, the outer hair cell damage score was statistically significantly high in Group 2 compared with Group 1. In addition, the outer hair cell damage score was also statistically significantly high in Group 2 compared with Group 3. Outer hair cell damage scores were low in Group 1 and Group 3, but there was no statistically significant difference between these groups. There was no statistically significant difference between the groups in terms of stria vascularis damage score examinations. Systemic ozone gas therapy is effective in the treatment of cell damage in cisplatin-induced ototoxicity. The intratympanic administration of ozone gas does not have any additional advantage over the rectal administration.
本研究旨在探讨直肠臭氧和鼓室内臭氧疗法对顺铂诱导的大鼠耳毒性的影响。18只雌性Wistar白化大鼠纳入本研究。所有大鼠的外耳道和鼓膜检查均正常。大鼠被随机分为三组。最初，所有大鼠均进行畸变产物耳声发射（DPOAE）测试，且发射测量正常。所有大鼠腹腔注射5 mg/kg/天顺铂，共3天。1周后经DPOAE证实，所有大鼠均出现耳毒性。直肠和鼓室内臭氧治疗组为第1组。第2组大鼠作为对照组未接受任何治疗。第3组大鼠接受直肠臭氧治疗。所有大鼠在全身麻醉下进行DPOAE测试，臭氧给药1周后全部处死进行病理检查。取出它们的耳蜗。检查外毛细胞损伤和血管纹损伤。在进行的统计分析中，根据DPOAE测试，第1组和第2组在所有频率上均观察到统计学上的显著差异。此外，在第2组和第3组之间，DPOAE测试也观察到统计学上的显著差异。然而，根据DPOAE测试，第1组和第3组之间未观察到统计学上的显著差异。根据组织病理学评分，与第1组相比，第2组外毛细胞损伤评分在统计学上显著更高。此外，与第3组相比，第2组外毛细胞损伤评分在统计学上也显著更高。第1组和第3组外毛细胞损伤评分较低，但两组之间无统计学上的显著差异。在血管纹损伤评分检查方面，各组之间无统计学上的显著差异。全身臭氧气体疗法对顺铂诱导的耳毒性中的细胞损伤治疗有效。鼓室内给予臭氧气体相对于直肠给药没有任何额外优势。
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