Esen Erkan, Özdoğan Fatih, Gürgen Seren Gülşen, Özel Halil Erdem, Başer Serdar, Genç Selahattin, Selçuk Adin
Clinic of Otolaryngology, Kocaeli Derince Training and Research Hospital, Kocaeli, Turkey.
Department of Histology and Embryology, Celal Bayar University School of Medicine, Manisa, Turkey.
J Int Adv Otol. 2018 Apr;14(1):22-26. doi: 10.5152/iao.2017.3137. Epub 2017 Jun 21.
The purpose of this study was to examine the anti-ototoxic impact of Ginkgo biloba extract and lycopene on the model of cisplatin-induced ototoxicity in rats.
Thirty-two Wistar albino rats were examined with the distortion product otoacoustic emission (DPOAE) test (MADSEN Capella2 ; GN Otometrics, ICS Medical, Chicago USA), and they were randomly divided into four groups. Group 1 (n=8) was defined as the healthy control group. Cisplatin was given intraperitoneally as single dose of 12 mg/kg to group 2 (n=8), group 3 (n=8), and group 4 (n=8). Group 2 was determined as ototoxic control group. G. biloba extract (100 mg/kg) was given to group 3, and 20 mg/kg lycopene was given to group 4 with orogastric feeding tube daily for 10 days. DPOAE test was repeated on day 10 on all the groups. Finally, histopathological examination was performed. The study has been lead in agreement with the principles by the Institutional Animal Care and Use Committee Review Board at Kocaeli University Medical Center (KOÜ HADYEK- 1/9-14). The animals were treated in accordance with protocols approved by this committee.
When DPOAE tests were compared, there was no significant difference in the four groups before the application (p > 0.05). At the end of day 10, in groups 2 to 4, statistically significant changes were observed (p < 0.05). According to the cisplatin group, a significant increase in the DP-grams on G. biloba and lycopene groups was observed (p < 00.5). Corti organ and spiral ganglion neurons of groups 1, 3, and 4 were observed to have weak expression. Strong reactions were determined in organum spirale and some spiral ganglions of the cisplatin group. The striae vascularis damage on group 2 was found to be more significant more compared with groups 3 and 4.
There is a protective effect of G. biloba and lycopene on cisplatin-dependent ototoxic rat model.
本研究旨在探讨银杏叶提取物和番茄红素对顺铂诱导的大鼠耳毒性模型的抗耳毒性作用。
采用畸变产物耳声发射（DPOAE）测试（MADSEN Capella2；GN Otometrics，ICS Medical，美国芝加哥）对32只Wistar白化大鼠进行检测，并将它们随机分为四组。第1组（n = 8）定义为健康对照组。第2组（n = 8）、第3组（n = 8）和第4组（n = 8）腹腔注射单剂量12 mg/kg顺铂。第2组被确定为耳毒性对照组。第3组给予银杏叶提取物（100 mg/kg），第4组通过灌胃饲管每日给予20 mg/kg番茄红素，持续10天。在第10天对所有组重复进行DPOAE测试。最后，进行组织病理学检查。本研究是在科贾埃利大学医学中心机构动物护理和使用委员会审查委员会（KOÜ HADYEK - 1/9 - 14）的原则指导下进行的。动物按照该委员会批准的方案进行处理。
比较DPOAE测试结果时，四组在应用前无显著差异（p > 0.05）。在第10天结束时，第2至4组观察到有统计学意义的变化（p < 0.05）。与顺铂组相比，银杏叶组和番茄红素组的DP图有显著增加（p < 0.05）。观察到第1组、第3组和第4组的柯蒂氏器和螺旋神经节神经元表达较弱。在顺铂组的螺旋器和一些螺旋神经节中发现有强烈反应。与第3组和第4组相比，第2组的血管纹损伤更为明显。
银杏叶和番茄红素对顺铂依赖性耳毒性大鼠模型具有保护作用。
