用于分析普通狨猴（Callithrix jacchus）中不同免疫细胞及其分布的交叉反应性单克隆抗体综合组。
Comprehensive panel of cross-reacting monoclonal antibodies for analysis of different immune cells and their distribution in the common marmoset (Callithrix jacchus).
作者信息
Neumann Berit, Shi Tingchuan, Gan Li Lin, Klippert Antonina, Daskalaki Maria, Stolte-Leeb Nicole, Stahl-Hennig Christiane
机构信息
Unit of Infection Models, German Primate Center, Leibniz Institute for Primate Research, Goettingen, Germany.
出版信息
J Med Primatol. 2016 Jun;45(3):139-46. doi: 10.1111/jmp.12216. Epub 2016 May 16.
BACKGROUND
Common marmosets are extensively used in immunological and pharmacological research, and the usage of methods such as flow cytometry gain increasing importance.
METHODS
Using multicolor flow cytometry cross-reactivity of monoclonal antibodies with cells of common marmosets was analyzed. Furthermore, frequencies of immune cells and immunological parameters were assessed in healthy common marmosets.
RESULTS
A total of 97 clones of monoclonal antibodies raised against CD markers, chemokine receptors, and miscellaneous markers were tested. Additionally, baseline frequencies of different innate and adaptive immune cells as well as certain parameters, such as activation and memory T-cell and B-cell distribution, are provided.
CONCLUSION
Our study gives an extended overview of cross-reactive antibodies for flow cytometric analysis of immune cells as well as baseline values for different immune parameters in healthy common marmosets.
背景
普通狨猴广泛应用于免疫学和药理学研究，流式细胞术等方法的使用愈发重要。
方法
采用多色流式细胞术分析单克隆抗体与普通狨猴细胞的交叉反应性。此外，还评估了健康普通狨猴体内免疫细胞的频率和免疫参数。
结果
共检测了97个针对CD标志物、趋化因子受体和其他标志物的单克隆抗体克隆。此外，还提供了不同先天性和适应性免疫细胞的基线频率以及某些参数，如活化和记忆T细胞及B细胞分布。
结论
我们的研究对用于免疫细胞流式细胞术分析的交叉反应抗体以及健康普通狨猴不同免疫参数的基线值进行了扩展概述。
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