Thean E T, Toh B H
Department of Pathology and Immunology, Monash Medical School, Prahran, Vic., Australia.
Immunol Cell Biol. 1989 Feb;67 ( Pt 1):41-8. doi: 10.1038/icb.1989.5.
Human alpha-lactalbumin (alpha-LA), a milk protein normally produced by, and restricted to, functionally differentiated breast epithelial cells has been shown immunohistochemically to be a good marker for breast cancer. Antibodies with specificity only for human alpha-LA have not previously been reported. The present study documents the production and characterization of an IgG1 murine monoclonal antibody with specificity restricted only to human alpha-LA. This monoclonal antibody, designated ET-1, was purified by affinity chromatography using human alpha-LA. Specificity of ET-1 for human alpha-LA was established by enzyme linked immunosorbent assay (ELISA), absorption studies, immunoprecipitation and by immunoblotting. ET-1 binds to human alpha-LA with a KD of 7.41 x 10(-8) mol/l and does not cross-react with bovine alpha-LA. This unique reactivity of ET-1, which is not inherently shared by polyclonal antisera, should enable the antibody to be used for the development of a sensitive immunoassay for circulating human alpha-LA.
人α-乳白蛋白（α-LA）是一种通常由功能分化的乳腺上皮细胞产生且仅局限于该细胞的乳蛋白，免疫组织化学显示它是乳腺癌的良好标志物。此前尚未报道过仅对人α-LA具有特异性的抗体。本研究记录了一种仅对人α-LA具有特异性的IgG1小鼠单克隆抗体的制备及特性。这种单克隆抗体命名为ET-1，通过使用人α-LA的亲和层析进行纯化。ET-1对人α-LA的特异性通过酶联免疫吸附测定（ELISA）、吸收研究、免疫沉淀和免疫印迹得以确定。ET-1与人α-LA结合的解离常数（KD）为7.41×10⁻⁸mol/L，且不与牛α-LA发生交叉反应。ET-1这种独特的反应性是多克隆抗血清所不具备的，这应能使该抗体用于开发一种检测循环中人α-LA的灵敏免疫测定法。
