Simícková M, Lang B A, Semotán K, Kocent A
Clin Chim Acta. 1987 Mar 30;163(3):257-65. doi: 10.1016/0009-8981(87)90244-0.
A sandwich non-competitive enzyme immunoassay for the measurement of human alpha-lactalbumin in serum, milk, and tumour tissue cytosol was developed using affinity-purified polyclonal antibody adsorbed to solid phase. The detection limits of this procedure in tubes (macromethod) and in wells of microtitre plates (micromethod) are 50 pg and 25 pg/sample, respectively, which means 2.5 micrograms/l of serum at appropriate dilution. The micromethod enables a reduction of the volumes used in the reaction with equal sensitivity, accuracy and reproducibility. A comparison of alpha-lactalbumin concentrations in serum of healthy, pregnant, and lactating women determined by our method with those measured formerly by radioimmunoassays proved the specificity of our procedure.
利用吸附于固相的亲和纯化多克隆抗体，开发了一种夹心非竞争性酶免疫测定法，用于测定血清、牛奶和肿瘤组织胞质溶胶中的人α-乳白蛋白。该方法在试管中（宏观方法）和微量滴定板孔中（微观方法）的检测限分别为50 pg/样品和25 pg/样品，这意味着在适当稀释时血清浓度为2.5微克/升。微观方法能够在同等灵敏度、准确度和重现性的情况下减少反应中使用的体积。将我们的方法测定的健康、怀孕和哺乳期妇女血清中的α-乳白蛋白浓度与以前通过放射免疫测定法测得的浓度进行比较，证明了我们方法的特异性。
