Mahajan Vivek, Gaymalov Zagit, Alakhova Daria, Gupta Richa, Zucker Irving H, Kabanov Alexander V
Division of Molecular Pharmaceutics, Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, NC 27599, USA; Department of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, USA.
Division of Molecular Pharmaceutics, Center for Nanotechnology in Drug Delivery, Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, NC 27599, USA; Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, College of Pharmacy, University of Nebraska Medical Center, Omaha, NE 68198-5850, USA.
Data Brief. 2016 Apr 1;7:1269-82. doi: 10.1016/j.dib.2016.03.087. eCollection 2016 Jun.
The data contains 14 figures supporting the research article "Horizontal gene transfer from macrophages to ischemic muscles upon delivery of naked DNA with Pluronic block copolymers" [1]. The data explains the surgical procedure and histological characterization of Murine Hind Limb Ischemia. The data also shows the kinetics of luciferase gene expression, spread of GFP expression through muscle and the colocalization of GFP with cellular markers in ischemic muscles injected with pDNA alone or pDNA/Pluronic. Finally the data shows the effect of Pluronic Block Copolymer to enhance total gene expression (cmv-promoter driven luciferase gene) in coculture of DNA transfected MØs with muscle cells.
这些数据包含14幅图，支持研究论文《使用普朗尼克嵌段共聚物递送裸DNA时巨噬细胞向缺血肌肉的水平基因转移》[1]。这些数据解释了小鼠后肢缺血的手术过程和组织学特征。这些数据还显示了荧光素酶基因表达的动力学、绿色荧光蛋白（GFP）表达在肌肉中的扩散以及单独注射pDNA或pDNA/普朗尼克的缺血肌肉中GFP与细胞标志物的共定位。最后，这些数据显示了普朗尼克嵌段共聚物在DNA转染的巨噬细胞与肌肉细胞共培养中增强总基因表达（巨细胞病毒启动子驱动的荧光素酶基因）的作用。
