Ahmad M A, Najmi A K, Mujeeb M, Akhtar M
Department of Pharmacology, Faculty of Pharmacy, Jamia Hamdard, New Delhi India.
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi India.
Drug Res (Stuttg). 2016 Aug;66(8):407-14. doi: 10.1055/s-0042-107787. Epub 2016 May 25.
The role of guggulipid was evaluated in high fat diet and middle cerebral artery occlusion (MCAO) induced ischemic cerebral dysfunctions in rats of either sex.
Ethyl acetate extract of guggul known as guggulipid was prepared and administered to rats. Animals were divided into 9 groups, consisting 6 rats, each receiving different treatments per orally for 8 weeks. Control group rats received normal control diet while rest of the other groups animals were fed high fat diet (HFD) for 8 weeks. Cerebral ischemia was induced for 2 h followed by reperfusion for 22 h. Locomotor activity and grip strength tests were performed immediately after 24 h of reperfusion followed by biochemical estimations and histopathology.
Locomotor activity and grip strength were significantly decreased in HFD and HFD fed MCAO groups and improved significantly in pretreatment groups. Cerebral infarction, thiobarbituric acid reactive substances (TBARs), nitric oxide and tumor necrosis factor alfa (TNFα) levels were increased, pretreatment of guggulipid alone and with aspirin significantly reduced these markers. Reduced glutathione (GSH), superoxide dismutase (SOD) and catalase, levels were decreased but all drug pretreated groups showed significant improvement in those markers.
Guggulipid demonstrated neuroprotection owing to its hypolipidemic, antioxidant, anti-inflammatory and anti-thrombotic activities but further research is warranted to confirm its role in cerebral ischemia.
评估古古勒脂对高脂饮食和大脑中动脉闭塞（MCAO）诱导的雌雄大鼠缺血性脑功能障碍的作用。
制备古古勒脂（已知的古古勒乙酸乙酯提取物）并给予大鼠。动物分为9组，每组6只，每组经口接受不同处理，持续8周。对照组大鼠给予正常对照饮食，其余各组动物给予高脂饮食（HFD）8周。诱导脑缺血2小时，随后再灌注22小时。再灌注24小时后立即进行运动活动和握力测试，随后进行生化评估和组织病理学检查。
高脂饮食组和高脂饮食喂养的MCAO组的运动活动和握力显著降低，预处理组则显著改善。脑梗死、硫代巴比妥酸反应性物质（TBARs）、一氧化氮和肿瘤坏死因子α（TNFα）水平升高，单独使用古古勒脂和与阿司匹林联合预处理可显著降低这些指标。还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶水平降低，但所有药物预处理组在这些指标上均有显著改善。
古古勒脂因其降血脂、抗氧化、抗炎和抗血栓形成活性而具有神经保护作用，但需要进一步研究以证实其在脑缺血中的作用。
