Lucas P A, Syftestad G T, Goldberg V M, Caplan A I
Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106.
J Biomed Mater Res. 1989 Apr;23(A1 Suppl):23-39. doi: 10.1002/jbm.820231306.
A controlled-release delivery vehicle for water-soluble osteogenic proteins from demineralized bone matrix was constructed using purified type I collagen. The water-soluble proteins were isolated from a 4 M GdnHCl extract of bone matrix. Although the water-soluble proteins were capable of inducing cartilage formation in vitro, they were incapable of inducing cartilage or bone in vivo when implanted intramuscularly into mice in the absence of an appropriate delivery vehicle. The collagen-based delivery vehicle alone was also incapable of inducing osteogenesis in vivo. However, when the water-soluble proteins were incorporated into the delivery vehicle, the combination was capable of inducing cartilage and bone 76% of the time. These results demonstrate that it is possible to formulate a controlled-release delivery vehicles for soluble bioactive factors which upon release interact with local responsive cells.
利用纯化的I型胶原蛋白构建了一种用于从脱矿骨基质中释放水溶性成骨蛋白的控释递送载体。水溶性蛋白从骨基质的4M盐酸胍提取物中分离得到。尽管这些水溶性蛋白在体外能够诱导软骨形成，但在没有合适递送载体的情况下，将其肌肉注射到小鼠体内时，它们在体内无法诱导软骨或骨形成。单独的基于胶原蛋白的递送载体在体内也不能诱导成骨。然而，当将水溶性蛋白掺入递送载体中时，这种组合有76%的时间能够诱导软骨和骨形成。这些结果表明，有可能为可溶性生物活性因子配制一种控释递送载体，该载体在释放后与局部反应性细胞相互作用。
