Gazdag M, Szepesi G
Chemical Works of Gedeon Richter, Ltd., Budapest Hungary.
J Chromatogr. 1989 Mar 3;464(2):279-88. doi: 10.1016/s0021-9673(00)94246-8.
Different analytical tasks in the pharmaceutical analysis can be classified according to the separation problems into three main groups: trace analysis, assay methods and separation of closely related compounds including isomers. The most important requirements of high-performance liquid chromatographic (HPLC) methods with respect of the separation problems are summarized. Considerations and recommendations for the selection of the most applicable HPLC system to solve particular analytical problems are discussed. HPLC methods can be compared on the basis of the system resolution (SR) and system selectivity (SS). Criteria developed for the characterization of HPLC methods considering the difficulties created by the different analytical problems are established. The principles of the selection of the most applicable separation systems are demonstrated through some practical examples in pharmaceutical analysis.
痕量分析、含量测定方法以及包括异构体在内的结构相近化合物的分离。总结了高效液相色谱（HPLC）方法在分离问题方面的最重要要求。讨论了选择最适用的HPLC系统以解决特定分析问题的考虑因素和建议。HPLC方法可基于系统分辨率（SR）和系统选择性（SS）进行比较。建立了考虑不同分析问题所带来困难的HPLC方法表征标准。通过药物分析中的一些实际例子展示了选择最适用分离系统的原则。
