Huang Yun, Ferrie John J, Chen Xing, Zhang Yitao, Szantai-Kis D Miklos, Chenoweth David M, Petersson E James
Department of Chemistry, University of Pennsylvania, 231 South 34th Street, Philadelphia, PA 19104, USA.
Chem Commun (Camb). 2016 Jun 14;52(50):7798-801. doi: 10.1039/c6cc00105j.
Thioamide residues can be effective, minimally-perturbing fluorescence quenching probes for studying protein folding and proteolysis. In order to increase the level of quenching, we have here explored the use of adjacent dithioamides. We have found that they are more effective fluorescence quenchers, as expected, but we have also observed unexpected changes in the thioamide absorption spectra that may arise from n-to-π* interactions of the thiocarbonyls. We have made use of the increased quenching to improve the fluorescence turn-on of thioamide protease sensors.
硫代酰胺残基可以作为有效的、对荧光干扰最小的猝灭探针，用于研究蛋白质折叠和蛋白水解。为了提高猝灭水平，我们在此探索了相邻二硫代酰胺的用途。正如预期的那样，我们发现它们是更有效的荧光猝灭剂，但我们也观察到硫代酰胺吸收光谱中出现了意想不到的变化，这可能是由硫羰基的n到π*相互作用引起的。我们利用增强的猝灭作用来改善硫代酰胺蛋白酶传感器的荧光开启。
