Shimada K, Oe T, Tanaka M, Nambara T
Pharmaceutical Institute, Tohoku University, Sendai, Japan.
J Chromatogr. 1989 Feb 24;487(2):247-55. doi: 10.1016/s0378-4347(00)83034-0.
Six reagents possessing ferrocene as an electrophore were prepared and evaluated for pre-column derivatization of amino compounds for their determination by high-performance liquid chromatography with electrochemical detection. The utility of these reagents was investigated employing phenethylamine as a model compound. Among these six, N-succinimidyl 3-ferrocenylpropionate was the best with respect to reactivity, stability and electrochemical properties. The developed method was applied to the determination of putrescine formed from ornithine by ornithine decarboxylase.
