Patchett M L, Monk C R, Daniel R M, Morgan H W
Department of Biological Sciences, University of Waikato, Hamilton, New Zealand.
J Chromatogr. 1988 Mar 18;425(2):269-76. doi: 10.1016/0378-4347(88)80031-8.
A method is presented for the pre-column derivatization of agmatine, arginine, citrulline or ornithine with o-phthalaldehyde-2-mercaptoethanol, and subsequent separation of the derivatives by reversed-phase liquid chromatography. Fluorescent response is linear from 10 to 150 pmol of injected analyte and detection limits range from 28 to 100 fmol. Response factors relative to the internal standard, homocysteic acid, were 1.16 (agmatine and arginine), 1.03 (citrulline) and 0.34 (ornithine). The applicability of the method to the measurement of arginase, arginine deaminase, arginine decarboxylase and other enzyme activities in bacterial extracts was examined.
本文介绍了一种用邻苯二甲醛-2-巯基乙醇对胍丁胺、精氨酸、瓜氨酸或鸟氨酸进行柱前衍生，随后通过反相液相色谱分离衍生物的方法。进样分析物10至150皮摩尔时荧光响应呈线性，检测限为28至100飞摩尔。相对于内标同型半胱氨酸的响应因子分别为：胍丁胺和精氨酸为1.16，瓜氨酸为1.03，鸟氨酸为0.34。考察了该方法在测定细菌提取物中精氨酸酶、精氨酸脱氨酶、精氨酸脱羧酶及其他酶活性方面的适用性。
