在原位杂交过程中，DNA探针会非特异性地与嗜酸性粒细胞结合：石炭酸铬变素可阻断与嗜酸性粒细胞的结合，但不抑制与特定核苷酸序列的杂交。
DNA probes bind non-specifically to eosinophils during in situ hybridization: carbol chromotrope blocks binding to eosinophils but does not inhibit hybridization to specific nucleotide sequences.
作者信息
Patterson S, Gross J, Webster A D
机构信息
Division of Immunological Medicine, Clinical Research Centre, Harrow, Middlesex, U.K.
出版信息
J Virol Methods. 1989 Feb;23(2):105-9. doi: 10.1016/0166-0934(89)90124-9.
PMID:2723016
Abstract
Eosinophils were found to bind radiolabelled DNA probes non-specifically during in situ hybridization. Pretreatment of cells with carbol chromotrope blocked non-specific binding without interfering with the recognition of specific nucleotide sequences.
摘要
在原位杂交过程中发现嗜酸性粒细胞会非特异性地结合放射性标记的DNA探针。用石炭酸品红对细胞进行预处理可阻断非特异性结合，且不会干扰对特定核苷酸序列的识别。
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