Toyohashi University of Technology, Electronics-Inspired Interdisciplinary Research Institute, Toyoh, Japan.
Toyohashi University of Technology, Department of Electrical and Electronic Information Engineering,, Japan.
J Biomed Opt. 2018 Feb;23(2):1-4. doi: 10.1117/1.JBO.23.2.020502.
Bundled fiber optics allow in vivo imaging at deep sites in a body. The intrinsic optical contrast detects detailed structures in blood vessels and organs. We developed a bundled-fiber-coupled endomicroscope, enabling stereoscopic three-dimensional (3-D) reflectance imaging with a multipositional illumination scheme. Two illumination sites were attached to obtain reflectance images with left and right illumination. Depth was estimated by the horizontal disparity between the two images under alternative illuminations and was calibrated by the targets with known depths. This depth reconstruction was applied to an animal model to obtain the 3-D structure of blood vessels of the cerebral cortex (Cereb cortex) and preputial gland (Pre gla). The 3-D endomicroscope could be instrumental to microlevel reflectance imaging, improving the precision in subjective depth perception, spatial orientation, and identification of anatomical structures.
束光纤允许在体内对深部部位进行成像。固有光学对比度可检测血管和器官的详细结构。我们开发了一种光纤束耦合内窥显微镜，通过多位置照明方案实现了立体三维（3-D）反射成像。两个照明点连接起来，以获得左右照明的反射图像。通过交替照明下两个图像之间的水平视差来估计深度，并通过具有已知深度的目标进行校准。该深度重建应用于动物模型，以获得大脑皮层（Cereb cortex）和包皮腺（Pre gla）血管的 3-D 结构。3-D 内窥显微镜可用于微观反射成像，提高主观深度感知、空间定向和解剖结构识别的精度。
