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免疫增强剂提高油乳剂灭活禽流感疫苗对鸡、鸭和鹅的效力。

Immunopotentiators Improve the Efficacy of Oil-Emulsion-Inactivated Avian Influenza Vaccine in Chickens, Ducks and Geese.

作者信息

Lu Jihu, Wu Peipei, Zhang Xuehua, Feng Lei, Dong Bin, Chu Xuan, Liu Xiufan, Peng Daxin, Liu Yuan, Ma Huailiang, Hou Jibo, Tang Yinghua

机构信息

National Research Center of Engineering and Technology for Veterinary Biologicals, Ministry of Agriculture, Key Laboratory of Veterinary Biological Engineering and Technology, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu, PR China.

College of Veterinary Medicine, Yangzhou University, Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, Jiangsu, PR China.

出版信息

PLoS One. 2016 May 27;11(5):e0156573. doi: 10.1371/journal.pone.0156573. eCollection 2016.

DOI:10.1371/journal.pone.0156573
PMID:27232188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4883754/
Abstract

Combination of CVCVA5 adjuvant and commercial avian influenza (AI) vaccine has been previously demonstrated to provide good protection against different AI viruses in chickens. In this study, we further investigated the protective immunity of CVCVA5-adjuvanted oil-emulsion inactivated AI vaccine in chickens, ducks and geese. Compared to the commercial H5 inactivated vaccine, the H5-CVCVA5 vaccine induced significantly higher titers of hemaglutinin inhibitory antibodies in three lines of broiler chickens and ducks, elongated the antibody persistence periods in geese, elevated the levels of cross serum neutralization antibody against different clade and subclade H5 AI viruses in chicken embryos. High levels of mucosal antibody were detected in chickens injected with the H5 or H9-CVCA5 vaccine. Furthermore, cellular immune response was markedly improved in terms of increasing the serum levels of cytokine interferon-γ and interleukine 4, promoting proliferation of splenocytes and upregulating cytotoxicity activity in both H5- and H9-CVCVA5 vaccinated chickens. Together, these results provide evidence that AI vaccines supplemented with CVCVA5 adjuvant is a promising approach for overcoming the limitation of vaccine strain specificity of protection.

摘要

先前已证明，CVCVA5佐剂与商用禽流感（AI）疫苗联合使用可在鸡体内提供针对不同AI病毒的良好保护。在本研究中，我们进一步研究了CVCVA5佐剂油乳剂灭活AI疫苗在鸡、鸭和鹅中的保护性免疫。与商用H5灭活疫苗相比，H5-CVCVA5疫苗在三个品系的肉鸡和鸭中诱导出显著更高滴度的血凝素抑制抗体，延长了鹅体内抗体的持续时间，提高了鸡胚中针对不同分支和亚分支H5 AI病毒的交叉血清中和抗体水平。在注射H5或H9-CVCA5疫苗的鸡中检测到高水平的黏膜抗体。此外，在H5-和H9-CVCVA5疫苗接种的鸡中，细胞免疫反应在增加细胞因子干扰素-γ和白细胞介素4的血清水平、促进脾细胞增殖以及上调细胞毒性活性方面均有显著改善。总之，这些结果证明，添加CVCVA5佐剂的AI疫苗是克服疫苗株特异性保护局限性的一种有前景的方法。

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