利用蚊细胞进行基孔肯雅病毒的传播

Propagation of Chikungunya Virus Using Mosquito Cells.

作者信息

Ang Swee Kim, Lam Shirley, Chu Justin Jang Hann

机构信息

Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University Health System, National University of Singapore, 5 Science Drive 2, Singapore, 117597, Singapore.

出版信息

Methods Mol Biol. 2016;1426:87-92. doi: 10.1007/978-1-4939-3618-2_8.

DOI:10.1007/978-1-4939-3618-2_8

PMID:27233263

Abstract

Chikungunya virus (CHIKV) is a mosquito-borne alphavirus that transmits in between a mosquito host vector to a primate host and then back to the mosquito host vector to complete its life cycle. Hence, CHIKV must be able to replicate in both host cellular systems that are genetically and biochemically distinct. The ability to grow and propagate the virus in high titers in the laboratory is fundamentally crucial in order to understand virus replication in different host cellular systems and many other CHIKV research areas. Here, we describe a method on CHIKV propagation using C6/36, a mosquito cell line derived from Aedes albopictus in both serum-containing and serum-free media.

摘要

基孔肯雅病毒（CHIKV）是一种蚊媒α病毒，在蚊虫宿主载体和灵长类宿主之间传播，然后再回到蚊虫宿主载体以完成其生命周期。因此，CHIKV必须能够在遗传和生化特性均不同的两种宿主细胞系统中进行复制。为了了解病毒在不同宿主细胞系统中的复制情况以及许多其他基孔肯雅病毒研究领域，在实验室中以高滴度培养和繁殖该病毒的能力至关重要。在此，我们描述了一种在含血清和无血清培养基中使用源自白纹伊蚊的蚊虫细胞系C6/36进行基孔肯雅病毒繁殖的方法。