Potiwat Rutcharin, Komalamisra Narumon, Thavara Usavadee, Tawatsin Apiwat, Siriyasatien Padet
Interdisciplinary Program of Biomedical Sciences, Faculty of the Graduate School, Chulalongkorn University, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 2011 Nov;42(6):1388-94.
Aedes albopictus C6/36 cell line was used to evaluate dengue virus serotype-3 (DENV-3) and chikungunya virus (CHIKV) co-infection. Virus infection was determined using a one-step duplex reverse transcriptase polymerase chain reaction (D- RT-PCR). D-RT-PCR was positive for both viruses when equal multiplicity of infection (MOI) was utilized. Co-infection with different titers between a higher CHIKV titer (MOI = 1.0) than DENV-3 titer (MOI = 0.1) showed similar results with that of equal titer. However, co-infection with a lower CHIKV titer (MOI = 0.1) than DENV-3 titer (MOI = 1.0) revealed positive results for only dengue virus infection, suggesting DENV competitive suppression of CHIKV. This competitive suppression occurred in mixed-infection samples but not in individually double infection (super infection) samples which produced both dengue and chikungunya virus progenies. Both virus replications depend on the amount of virus titer rather than serial infection. These findings have provided information regarding pathogen-pathogen interaction in host cell, which could be used to predict outbreaks, and to develop virus detection and vector control.
白纹伊蚊C6/36细胞系用于评估登革病毒3型（DENV-3）和基孔肯雅病毒（CHIKV）的共感染情况。使用一步双重逆转录聚合酶链反应（D-RT-PCR）来确定病毒感染。当使用相同的感染复数（MOI）时，两种病毒的D-RT-PCR均呈阳性。较高的基孔肯雅病毒滴度（MOI = 1.0）与较低的登革病毒3型滴度（MOI = 0.1）之间的不同滴度共感染显示出与相同滴度共感染相似的结果。然而，较低的基孔肯雅病毒滴度（MOI = 0.1）与较高的登革病毒3型滴度（MOI = 1.0）共感染仅显示登革病毒感染呈阳性结果，表明登革病毒对基孔肯雅病毒有竞争性抑制作用。这种竞争性抑制发生在混合感染样本中，但在产生登革病毒和基孔肯雅病毒子代的单独双重感染（重复感染）样本中未发生。两种病毒的复制均取决于病毒滴度的数量而非连续感染。这些发现提供了有关宿主细胞中病原体-病原体相互作用的信息，可用于预测疫情爆发，并用于开发病毒检测和病媒控制方法。
